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1、棉花学报CottonScience2015袁27渊2冤院104―110半野生棉棉仁含油量与SSR标记的关联分析111111李晓娜袁蔡小彦袁KiflomWelduOkubazghi袁王星星袁王春英袁刘方袁12121,3*1*周忠丽袁李诚袁王玉红袁祝水金袁彭仁海袁王坤波渊1.中国农业科学院棉花研究所/棉花生物学国家重点实验室袁河南安阳455000曰2.浙江大学农业与生物技术学院袁浙江杭州310058曰3.安阳工学院袁河南安阳455000曰冤摘要院为探究半野生棉群体材料的遗传变异和群体结构袁挖掘与含油量相关的标记位点袁
2、利用215对SSR引物袁对89份半野生棉自然群体进行遗传多样性尧群体结构和亲缘关系分析袁并进行棉仁含油量性状与标记的关联分析遥结果表明袁位点多态信息含量渊PIC冤为0.0222～0.7390袁平均为0.3452曰基因型多样性渊H忆冤介于0.0616～1.4412袁平均为0.5614遥群体结构分析将89份材料分为2个亚群袁群体结构简单袁遗传变异比较丰富袁可以用于半野生棉目标性状的关联分析遥应用关联分析袁在3个年份环境下重复检测出12个与棉仁含油量显著相关的位点渊＜0.05冤袁这12个位点可能与含油量性状存在稳定的关
3、联袁可为棉仁含油量性状的分子标记辅助选择提供依据袁为开展半野生棉其它性状的关联分析提供参考遥阔叶棉141平均棉仁含油量为41.90%袁为高油份含量品种的遗传改良提供理想材料遥关键词院半野生棉曰含油量曰关联分析中图分类号院S562.035文献标志码院A文章编号院1002-7807渊2015冤02-0104-07院10.11963/issn.1002-7807.201502002111111LiXiaona,CaiXiaoyan,KiflomWelduOkubazghi,WangXingxing,WangChunyi
4、ng,LiuFang,Zhou12121,3*1*Zhongli,LiCheng,WangYuhong,ZhuShuijin,PengRenhai,WangKunbo(1.455000,;2.310058,曰3.455000,)Thestudyobjectivesweretoestimategeneticdiversity,populationstructure,andidentifylociassociatedwithcotton-seedoilcontentinL.Todothis,89L.racesacce
5、ssions,and215pairsofSSRmolecularmark-erswerecompared.Geneticdiversity,populationstructure,andassociationmappinganalysiswereconductedusingdifferentsoftwarepackages.TheresultsshowedthatthepolymorphisminformationcontentoftheSSRmarkersrangedfrom0.0222to0.7390with
6、ameanof0.3452.TheShannon-Wienerdiversityindex(H')rangedfrom0.0616to1.4412withameanof0.5614.Populationstructureanalysisdividedthe89accessionsintotwosubpopulations.The89accessionshadextensivegeneticvaria-tionandasimplepopulationstructure,whichrevealedthenatural
7、populationtoberepresentativeforfurtherassociationanalysisofquantitativetraitsincotton.Associationanalysisshowedthat12markersweresignificantlyassociatedwithcottonseedoilcontent(＜0.05)over3successiveyears.Ofalltheaccessions,LAT141hadthehighestaveragecottonseedK
8、erneloilcontent(41.90%),revealingitspotentialtobeexploitedasagoodsourceofhighoilcontent.Thefindingsofthisstudycouldbeusedingeneticimprovementofcottonseedoilcontentofcultivatedspeciesthrou
