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1、中国科技论文在线http://www.paper.edu.cn参芪解郁方对产后抑郁大鼠Treg/Th17平#衡的干预作用研究1211**曲淼，李净娅，赵瑞珍，唐启盛5（1.北京中医药大学第三附属医院,北京100029；2.浙江省中医院,浙江杭州310006）摘要：目的：观察产后抑郁症模型大鼠Treg/Th17细胞比例的变化，阐明参芪解郁方对Treg/Th17平衡的干预作用。方法：SD雌性大鼠随机分为正常组、假手术、模型组、中药组10和西药组，分设灌胃1、2、4周三个时点。采用流式细胞术检测三个时点各组大鼠外周血Treg细胞和Th17细胞比例，并计算Treg
2、/Th17比值。结果：1w、2w模型组Treg/Th17较正常组明显升高（P<0.01），而4w模型组Treg/Th17较正常组明显降低（P<0.01），4w中药组较模型组升高明显（P<0.01）。结论：参芪解郁方可通过调控Treg/Th17的失衡状态发挥抗抑郁作用。15关键词：产后抑郁症；参芪解郁方；Treg/Th17平衡中图分类号：R285.5ImmunedysregulationonregulatoryTcellsandTh17cellsinpostpartumdepressionandtherolesof20Shen-Qi-Jie-Yu-Fang1
3、211QuMiao,LiJingya,ZhaoRuizhen,TangQisheng(1.ThirdaffiliatedhospitalofBeijingUniversityofChineseMedicine,Beijing100029;2.ZhejiangProvicialHospitalofTCM,Hangzhou310006)Abstract:IObjective:Shen-Qi-Jie-Yu-Fang(SJFang),establishedbyourteamleaderDr.Tang,was25oneoftheusefultherapyofpost
4、partumdepression(PPD)inTraditionalChineseMedicine(TCM).SJFangwastonifyingdeficiency,removingbloodstasis,nourishingbrainandinducingresuscitation.However,theimpactofimmunesystemofSJFangonPPDwasstillunknown.So,thepresentstudywasdesignedtoexploretheimmunomodulationeffectofSJFangonregu
5、latoryTcellsandTh17cells.MaterialsandMethods:NinetyFemaleSprague-Dawleyratsweredividedintofivegroupsrandomly.30PPDratsweremodelingwithhormonesimulatedpregnancy(HSP).RatsinPPDgroupwereovariecImmunedysregulationonregulatoryTcellsandTh17cellsinpostpartumdepressionandtherolesofShen-Qi
6、-Jie-Yu-Fangtomizedbilaterally,butratsinshamgroupwereexteriorizedwithassociatedfatpadnearovary.Theninjectionofprogesteroneandestradiolbenzoatewasadministeredfor23days.Onday24,theinjectionswereterminatedtoinitiatehormonewithdrawal.AfterHSP35modelprocedure,ratsweregavageadministrate
7、dfor1week,2weeksand4weeks.Immediately,behavioralassessmentsofsucroseconsumptionandforcedswimtest(FST)wereconducted.ImmuneimbalancestatuswasobservedinanalyzinglevelsofregulatoryTcellsandTh17cellsinperipheralbloodmononuclearcellswithflowcytometry.Results:TheresultssuggestedthatTregc
8、ellsdepletionmodulatedalterations
