不同途径输注造血干细胞对小鼠移植效果的比较_论文

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1、不同途径输注造血干细胞对小鼠移植效果的比较作者：蔡耘，黄绍良，黄科，陈惠芹，张绪超【摘要】为比较骨髓腔内（IBM）或静脉（IV）等不同途径输注小鼠造血干细胞后在受体内的分布特点及对移植效果的影响，将Ｃ57ＢＬ/6胎鼠及新生鼠外周血(FNPB)单个核细胞（MNC）移植经亚致死量60Coγ射线辐照的BALB/ｃ鼠。受鼠随机分为6组：单侧IBM组；双侧IBM组；IV组；双侧IBM+IV组；照射对照组；空白对照组。用组织冰冻切片和流式细胞术动态了解CFSE标记的FNPBMNC在受体内的分布变化，观察移植后受鼠生存状况、植入水平、造血恢复及GVHD情况。结果显示：19/19

2、IBM注射的供体FNPBMNC主要积聚于注射侧骨髓腔内，少量FNPBMNC可经血循环二次归巢，肺部滞留很少。单/双侧IBM组输注的FNPBMNC总数无明显差异，但经双侧IBM输入FNPBMNC渗漏至外周血或其它组织器官的比例更少；IBM各组造血重建速度均优于IV组，尤以双侧IBM组最快，其外周血象和造血干祖细胞集落产率在移植后第21天已接近正常水平，单侧IBM组、双侧IBM+IV组次之；IBM组各时点植入水平均明显高于IV组，双侧IBM组注射侧胫骨植入水平与单侧IBM组无明显差异，二次移植中双侧IBM组的植入水平明显高于IV组；IBM移植组90天存活率≥80%，I

3、V组仅为50%。结论：双侧IBM有利于更多造血干细胞归巢，促进植入和造血重建，提高IBM途径移植的效果。【关键词】骨髓腔内输注移植ComparisonofEfficaciesofAllogeneicHematopoieticStemCellTransplantationsbetweenDifferentRoutesofAdministrationinMiceAbstractWhenhematopoieticstemcells（HSCs）wereadministratedbyintravenousinfusion(IV),mostofthemweretrappedi

4、nsomenonhematopoieticorgansaslikelungsthathadabundantbloodcapillaries.Onlyasmallfractionofinjectedcellscouldhometothebonemarrow,whichreducedtheengraftmentofHSCs.Thepurposeofintrabonemarrow(IBM)transplantationwastofacilitatethehomingofHSCsdirectly.Basedontheestablishedmurinemodelforal

5、logeneicumbilicalcordbloodtransplantation(UCBT)byIBMinjection,theobjectiveofthisstudywastocomparethedistributionoffetalandneonatalperipheralblood(FNPB)mononuclearcells(MNC)invivoandthe19/19efficacyofHSCTbydifferentroutesofadministrationinmice.BALB/crecipientmiceexposedtosublethaldose6

6、0CoγrayweretransplantedwithFNPBMNCsfromＣ57BL/6mice.Recipientmiceweredividedintosixgroupsatrandom:unilateralIBMgroup;bilateralIBMgroup;IVgroup;bilateralIBM+IVgroup;irradiatedcontrolgroupandnormalgroup.ThedistributionofCFSElabeledFNPBMNCsintherecipientswasobservedinfrozensectionsof

7、differentorgansorbyflowcytometry.Thesurvivalrate,engraftmentlevel,recoveryofhematopoieticfunctionandGVHDofrecipientmicewerestudied.TheresultsshowedthatinfusedbyIBMroute,FNPBMNCsmainlyaccumulatedinthebonemarrow(BM)cavityoftheinjectedsidetibia.SomeofthemcouldentertheBMofnoninjectedbones

8、viabl