资源描述：
《isolation and identification of lipase producing thermophilic geobacillus》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、JournalofBioscienceandBioengineeringVOL.111No.3,272–278,2011www.elsevier.com/locate/jbioscIsolationandidentificationoflipaseproducingthermophilicGeobacillussp.SBS-4S:CloningandcharacterizationofthelipaseMuhammadTayyab,1NaeemRashid,1,⁎andMuhammadAkhtar1,21SchoolofBiologicalSciences,
2、UniversityofthePunjab,Quaid-e-AzamCampus,Lahore,54590,PakistanandSchoolofBiologicalSciences,2UniversityofSouthampton,Southampton,SO167PX,UKReceived1October2010;accepted19November2010Availableonline24December2010Athermophilicmicroorganism,SBS-4S,wasisolatedfromahotspringlocatedinGil
3、git,NorthernAreasofPakistan.Itwasfoundtobeanaerobic,gram-positive,rod-shaped,thermophilicbacteriumthatgrewonvarioussugars,carboxylicacidsandhydrocarbonsattemperaturesbetween45°Cand75°C.Complete16SrRNAgenesequenceofthemicroorganismexhibitedhomologytovariousspeciesofgenusGeobacillus.
4、Ahighesthomologyof99.8%wasfoundwithGeobacilluskaustophilus.Apartial(0.7kbp)chaperoningenesequencealsoshowedahighesthomologyof99.4%tothatofG.kaustophiluswhereasbiochemicalcharacteristicsofthemicroorganismweresimilartoGeobacillusuzenensis.Basedonbiochemicalcharacterization,16SrRNAand
5、chaperoningenesequences,weidentifiedSBS-4SasastrainofgenusGeobacillus.StrainSBS-4Sproducedseveralextracellularenzymesincludingamylase,proteaseandlipase.Thelipaseencodinggenewascloned,expressedinEscherichiacoliandthegeneproductwascharacterized.Therecombinantlipasewasoptimallyactivea
6、t60°C+2withstabilityatwidepHrange(6–12).TheenzymeactivitywasenhancedremarkablyinthepresenceofCa.TheKmandthe−1−1Vmaxforthehydrolysisofp-nitrophenylacetatewere3.8mMand2273μmolminmg,respectively.TheabilityoftherecombinantenzymetobestableatawidepHrangemakesitapotentialcandidateforusein
7、industry.©2010,TheSocietyforBiotechnology,Japan.Allrightsreserved.[Keywords:16SrRNA;Chaperonin;GeobacillusSBS-4S;Thermophiles;Thermostablelipase]Thermophilesprefertoliveathightemperatureswheremostoflipases.However,lowexpressionleveloftheseproteinsisahurdleintheotherorganismsfailtos
8、urvive.Therehasbeenincreas
