资源描述：
《immunomodulatory effects of secondary metabolites from thermophilic》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、Fish&ShellfishImmunology30(2011)1331e1338ContentslistsavailableatScienceDirectFish&ShellfishImmunologyjournalhomepage:www.elsevier.com/locate/fsiImmunomodulatoryeffectsofsecondarymetabolitesfromthermophilicAnoxybacilluskamchatkensisXA-1oncarp,Cyprinuscarpioa,*aaaGao-XueWang,YongWang
2、,Zong-FanWu,Hai-FengJiang,baa,**Rui-QiangDong,Fu-YuanLi,Xiao-LinLiuaNorthwestA&FUniversity,XinongRoad22nd,Yangling,Shaanxi712100,ChinabDeyangCenterforDiseaseControlandPrevention,MinjiangxiRoad391st,Deyang,Sichuan618000,ChinaarticleinfoabstractArticlehistory:Abacterialstrainwithput
3、ativeimmunomodulatorypropertieswasisolatedfromXi’anhotspringsinReceived23October2010China.Comparisonof16SrRNAgenerevealeda97%similaritybetweenthetestedstrain(designatedReceivedinrevisedformXA-1)andAnoxybacilluskamchatkensis.Twocompoundsisolatedfromthesecondarymetabolitesof12March2
4、011XA-1wereidentifiedbyspectraldata(infrared,nuclearmagneticresonanceandmassspectrometry)as:Accepted12March2011(1)cyclo(Gly-L-Pro)and(2)cyclo(L-Ala-4-hydroxyl-L-Pro).TwocyclicdipeptidesshowedstimulatoryAvailableonline21March20111propertiestowardsarangeofparameterswhenadoseof20mgkg
5、bodyweightwasintraperitoneallyinjectedinnaivecommoncarp,Cyprinuscarpio.Innateimmuneparameters(serumSOD,lysozymeandKeywords:bactericidalactivity,andphagocyticactivitybyperipheralbloodleucocytes)alongwiththeexpressionofImmunomodulatoryeffectCyprinuscarpiotwoimmune-relatedgenes(IL-1b
6、andiNOS)inbloodwereexaminedafter7,14,21,and28daysofInnateimmuneparameterinjection.Intheabsenceofinfection,immunomodulatorsshouldideallynotaffectnormalphysiologySecondarymetabolitesandimmunityofthehost;possiblenegativeoutcomesofactivatedimmuneresponsesinthenaivestateThermophilesare
7、discussed.Protectionbytwobacterialdipeptideswasassessedinanintraperitonealinjectionchal-lengetrialwithliveAeromonashydrophila.Bothcompoundsreducedmortality,withthehighestsurvivalrateobservedinthegroupthatreceivedcompound2(80%)followedbythegroupthatreceivedcompound1(65%)whilecontro
8、lgroupscoredtheworse(15%).Elucida
