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1、AgriculturalSciencesinChina2011,10(9):1346-1353September2011AGeneticLinkageMapofKenaf(HibiscuscannabinusL.)BasedonSRAP,ISSRandRAPDMarkersZHANGGuang-qing1,2,QIJian-min1,3,ZHANGXiao-chen1,FNAGPing-ping1,SUJian-guang4,TAOAi-fen1,LANTao1,WUWei-ren1,3andLIUAi-min51KeyLaboratoryforGenetics,B
2、reedingandMultipleUtilizationofCrops,MinistryofEducation,Fuzhou350002,P.R.China2CropSciencesInstitute,FujianAcademyofAgriculturalSciences,Fuzhou350013,P.R.China3CollegeofLifeScience,FujianAgricultureandForestryUniversity,Fuzhou350002,P.R.China4InstituteofBastFiberCrops,ChineseAcademyof
3、AgriculturalSciences,Changsha410205,P.R.China5DepartmentofChemistryandCenterforMetalsinBiocatalysis,UniversityofMinnesota,St.Paul,MN55108,USAAbstractKenaf(HibiscuscannabinusL.)isoneofthemosteconomicallyimportantcropsfornon-woodfiberproduction.Theobjectiveofthisstudywastoestablishagenet
4、iclinkagemapofkenafwithhigherdensityofmolecularmarkers.Asemi-wildvarietyGa42andacultivarAlainkenafwereusedasparentstoconstructanFpopulationconsistingof155plants.2Thegeneticlinkagemapcomprising134markerlociwasconstructed,including65sequence-relatedamplifiedpolymorphism(SRAP),56inter-sim
5、plesequencerepeat(ISSR),and13randomlyamplifiedpolymorphicDNA(RAPD)markers.Thismapspans2108.9cMandcontains20linkagegroupswithanaveragemarkerdensityof15.7cMbetweentheadjacentmarkers.Keywords:kenaf,geneticmap,SRAP,ISSR,RAPDAsoneofthemosteconomicallyimportantcropsINTRODUCTIONforfoodornon-w
6、oodfiberproduction,kenafismainlyusedasasourceoffiberformakingropes,sacks,KenafisamemberoftheHibiscusintheMalvaceaecanvas,andcarpets(Dempsey1975;Lietal.1990).familyandnativetoeast-centralAfrica,whereithasHowever,newvalue-addedapplicationsofkenafhavebeengrownforseveralthousandyearsforfoo
7、dandbeendevelopedsuchas,pulpingandpapermaking,oilfiber(Coetzee2004).Thediversifiedformsofthekenafabsorption,bioremediation,particleboard,filtrationspeciesanditsrelativespeciesinHibiscusgenus,in-media,andanimalfeed(SellersandReichert1999;cludingroselle,arefoundgrowingwidelyinmanycoun-
