SN-T 1200-2003 烟草中转基因成分定性PCR检测方法

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1、ProtocolofqualitativePCRforthedetectionofgeneticallymodifiedcomponentsintobaccoa3=.3.2.13TrisItris(hydroxymethyl)arninomethane,Z(%4S)JfilSEflti2a3.2.14DNAIde~x~ribonucleicacid,li##@#$I@.3.2.15dNTPs1dTTP,dATP,dCTP,dGTP.3.2.16dTTPIdeoxyribonucleosidetriphosphate,M#@VZ#l@3.2.17dATPldeoxyadenosinetriph

2、osphate,ll#@BP3@Ra3.2.18dCTPldeoxycytidlnetriphosphate,W#lekB3fEl#l@.3.2.19dGTPldeoxyguanosinekphosphate,%%4~~@@3.2.20dUTPIdeoxyuridinetriphoaphate,lR#R%Z119B!.3.2.21bptbasepair,&&Xlf.fQaH&#s&At7SH##&&#tlP#$d@&W7&sb.k9'rcH&

3、;amp;dBflBA,#&BJ&$$8.$!N@###aH&&*&?@&&@#BekQ,#&.&@&#,BR*&&fl,P;f*I%#.RH&3244fi~&~Rs~&a~~&&~~~*Am~*&~~~~mHm~m~m~~&~~~s&~~#~~~sw~~R-+%sm~B~~~

4、~~#+~~~~B~~~~k?uA~#~m~~~&%%~fiRMB#&ESHaHRSB@BM&N&SHB@HH@@nBaF*&k?,B#BeSMSfi&B~##ESMbC#LiSH&;J$BBUWaN'F#SBM5619S8'H83iM@J3=91%i35sG&?,NOs@kPNB#SESB;NPT-I1r'#!&!,i$lbbkrtfal$&IEI&SiATMV-

5、CP,CMV-CP,TMV-54KD,RT,PVY-CP.6.1*B?@miH$&M8GB/T668243~~7JC(Z%*)M)5#5.6.2-Fn~Bl~*~jifQ~#~~~~~~~N~6,2.1akB,Z~~B.#r#gt,CTAB,%%,EDTA,Tris,BQ@,P%~iFiLe6.2.2dNTPa*TaqDNAfltf3sBC,bl~#f~t#~ZBi!,DNAj).?.it6lE.&%4&#@?4%.6.3X#ZPE@Ylllkn(%%1).1BR90SBatarllA,*ZRS~IJiR3I#Afla~126R&amp

6、;851?RH91¶b@PJFtll#&.tf;BbB%5'-gctcctacaaatgccatca-3'35s9Cn19%~Blk39t;llW5'-grtagtgggattgtgcgtca-3'5'-gaatcctgttgccggtcttg3'NOS)hi#.l8obp%B!hl5'-ttatcctagtttgcgcgcta-3'5'-ggatctcctgtcatct-3'NPT-I19bM17

7、3bpMIY11115'-gatcatcctgatcgac-3'5'-cc~actagttaacaatttgatt~ga-3'Btfim-299bp%Yr$l!&M5'-ccggaag~tttaggact~ta~gt-3'5'-aagacgttggcagctggtcg3'CMVCPmu202bpPS!Sbtl5'-ctcgaatttgaatgcgcgaa-3'TrisItris(hydroxymet

8、hyl)aminomethane,3(%qgoDNAIdeoxyribonucleicacid,li##.$I###@.dNTPsIdTTP,dATP,dCTP,dGTP.dTTPIdeoxyribonucleoaidetriphosphate,NRll$%f:3@@.dATPIdeoxyadenosinetriphosphate,j3jt%#l$Z@B.dCTPtdeoxycytidinetriphosphate,W#$Mft:Z@Bp.dCTPIdeoxyguanosinetl*iphosphate,W#&$P3@@.dUTPsdeoxyuridinetriphospha

9、te,N#R~G~@.bp,basepair,MSX$.R~E~Q%~~T$BrH#WBrJ~&~PR*~~SH&+~BE1SBB~3A~XSHS+.$QbENSiri&amp