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1、SNI/CCI/改良CCI大鼠模型疼痛行为学特点和规律比较林露1聂发传2易斌2陶国才1(第三军医大学西南医院麻醉科，重庆，400038) 【摘要】 目的：比较慢性坐骨神经缩窄损伤(CCI)、改良慢性坐骨神经缩窄损伤(iCCI)和坐骨神经分支选择性损伤模型(SNI)的疼痛规律和特征。方法：选择150～200g的SD大鼠40只,随机分为四组(Sham组、CCI组、iCCI组、SNI组，n=10)，对照组仅暴露坐骨神经，后立即缝合，CCI组、iCCI组和SNI组分别于右后肢制作CCI、SNL、SNI疼痛模型。术前至术
2、后1d，3d，7d，14d，21d使用机械和热辐射刺激测定术侧后趾,比较它们对疼痛刺激的反应差异。结果：Sham组术前后机械/热缩足阈值没有明显差异(P>0.05)。CCI和iCCI组在术后第7天即出现明显的热/机械痛阈降低，并在术后第14天到达低谷(P<0.05)。iCCI组比CCI痛阈下降更加显著(P<0.05)。SNI疼痛模型对机械刺激敏感而对热刺激不敏感。结论：三种神经病理性疼痛模型的疼痛规律和特征并不相同，应根据实验设计选择适当的模型，并在同一系列研究中采用同一模型。【关键词】神经病理性疼痛；动物模型
3、；外周神经损伤ComparisionoftheprinciplesandcharacteristicsofpainproceedingbetweentheSNI,CCIandimprovedCCImodels.LinLu1,NieFa-chuan2,YiBin2,TaoGuo-cai1.(DepartmentofAnesthesia,SouthwestHospita1,ThirdMilitaryMedicalUniversity,Chongqing400038,China)【Abstract】 Objectiv
4、e:Themodelsofperipheralspinalnerveinjurycanbeclassifiedassparednerveinjurymodel(SNI)、chronicconstrictioninjuryofthesciaticnervemodel(CCI)、andimprovedchronicconstrictioninjuryofthesciaticnervemodel(ICCI),etal.Tocomparetheprinciplesandcharacteristicsofpainproc
5、eedingafterperipheralspinalnerveinjurybetweenthethreemodels.Methods:FortyfemaleSDrats(weightedfrom150to200g)wererandomizedintofourgroups(groupSham,groupCCI,groupiCCI,groupSNI,n=10).ThesciaticnerveoftherighthindlegwasexposedwithoutanyinjuryingroupSham,butoper
6、atedaccordingtothemethodsdescribedfollowingingroupCCI,groupiCCIandgroupSNI.Thenappraisalingthedegreeofneuropathicpainbythescoresof50%pawwithdrawthresholdsandradiantheatstimulationbeforethesurgeryand1,3,7,14,21dayslater.Results:Duringexperiments,anysignofmech
7、anicalorheatallodyniadidnotoccuringroupSham.ButthesurgicalprocedureingroupCCIandiCCIproducedanapparentandstablehyperalgesiatonoxiousheatandmechanicalstimulationsincethe7thdayafteroperation,andreachedthelowestpeakonthe14thdaybothly.Butthedegressionofpainthres
8、holdwasmoreresponsiveingroupiCCIthangroupCCIafterthe7thdayofthesurgery.TheSNIgroupshowedinsensitivetoheatstimulationbutmoresensitivetothemechanicalstimulationthangroupCCIandiCCI.Conclusion:Thepr
