生物分离工程-双语版-4知识讲解.ppt

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1、生物分离工程-双语版-4IintroductionProteininterfacialadsorptionLowproteinconcentration(nobarrier)Highsurfaceproteinconcentration(barrier)AelectrostaticBstericCosmoticeffectProteinsadsorptiononGas-liquidinterface(1)enzymeadsorbedunfoldedpartiallyactiveorinactivestateasrigid

2、film(2)proteinsunfoldingdependsonconformationalstability(3)interfacesareprimarilyresponsibleforproteininactivation(4)Shearassociateddamageisseverewhengas-liquidinterfacepresentApumpBcentrifugesCultrafiltration(5)proteinsonadsorptionatfluidinterfacesundergoachange

3、fromtheirglobularconfigurationtoanextendedchainstructure(6)changeofproteinstructureleadtoactivitychangesProteinsadsorption1proteinmoleculesinaqueoussolutionhaveahydrophobicinteriorandahydrophilicexterior2structuralperturbation-rearrangementsinvolvingchangesininte

4、ratomicdistanceaffecttheintermolecularvanderwaalsinteraction3chargegroupsintheapolarinterioroftheproteinmoleculetendtoformionpairs.4intramolecularpeptidehydrogenbondsaremorefavorablethanpeptide-waterinteraction5thevariationsinthehydrogenbonding,ionpairing,hydroph

5、obicinteractionaffecttherotationalmotionProteinsadsorption6Increaseofentropyoriginatingfromthedehydrationoftheinterface-surface,anddehydrationofstructuralchangesarethedrivingforce.7pHandshakingsignificantlyaffecttherateoftransportofproteintointerface.8Surfactants

6、occupythesurfaceandpreventtheunfoldinganddenaturationofprotein.9ProteinsadsorbedexistmultiplebindingmodestatesAweaklyandtightlyboundproteinsBNotallofadsorbedproteinisremovedunderoneoraspecifiedsetofconditionsIIReactionandinactivationatliquid-liquidinterfaces1lipi

7、d-waterinterfacesAInhibitoryproteinpreventsthelipasefrombindingBSerumalbuminandβ-lactoglobulinresultinlipaseactivitylossof93and92%CDifferentconformationalstatesexhibitdifferentactivitiesandactivitiesofthisproteincouldberegulated.BAqueoustwo-phasesystems(1)Moderat

8、eselectivity(2)highyield(3)Biocompatibility(4)amenabilitytoscale-up(5)Electrostaticinteractionplayasignificantpartinproteinadsorptionandinteractionattheinterfa