生物分离工程-双语版-7讲课教案.ppt

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1、生物分离工程-双语版-7Someparametersofinfluencingproteins-surfaceinteraction1Electrostaticinteractions2pH3Surfacecharge4Co-adsorptionoflow-molecularweightions5Isoelectricpoint6Intermolecularforces(1)molecules-molecules(2)solvent-solventinteraction(3)strengthoffunctionalgroupbonds(4)chemistryofsolidsur

2、faceandmorphologyProteinsinsolutiondiffusetointerface1Thermodynamicallyfavorableprocess2Conformationalhydrationenergylostatinterface.3Initially,atlowproteinconcentration,nobarrieradsorption,diffusioncontrolled4Athighsurfaceconcentration,activationenergybarrieradsorption(1)Electrostatic(2)St

3、eric(3)Osmoticeffectclosetotheinterfacialorsurfacelayers(4)RearrangeatsurfaceisratedeterminingAdsorptionofproteinsatinterface1Proteinsatgas-liquidinterfacesareinanunfoldpartiallyactiveorinactivestate(1)Conformationalstabilityofflexiblesegments(2)Interfacesareprimarilyresponsibleforproteinina

4、ctivation(3)Shear-associateddamageissevereatgas-liquidinterfaceApumpBcentrifugesCultrafiltrationAdsorptionofproteinsatinterface2Proteinsundergoachangefromglobularconformationtoanextendedchain3Adsorptionofproteins-enzymeatinterfaceisacomplexphenomenon(1)transporttotheinterfacebydiffusion,conv

5、ection,mixingandshearingaction(2)adsorption-desorptionatinterfaceAinterfacialchemicalreactionBkineticadsorptionanddesorptionmechanisms(3)structuralchangeofmoleculesincontactwithinterface(4)adsorptioncompetitionbetweenmoleculesofdifferentnatureormolecularweightFactorsofinfluencingproteinadsor

6、ptionontosurface1Proteinadsorptionkinetics2Chemicalequilibriumbetweeninterfacialproteinandsolutionproteins3Flowofproteinpastadsorbingsurface4ConformationofproteinsintheadsorbedlayerExample7.11Somepositiveinfluenceorconsequences(1)stabilizationofmicroemulsion,pharmaceuticalcreams,lotions,form

7、ulatedfoodsandfoams(2)proteinpurificationstrategydevelopments(3)drugdeliverysystems(4)biosensors2Somenegativeaspectsofproteinadsorption(1)thrombusdevelopmentonbloodvesselsandinartificialimplantmaterials(2)foulingofkidneydialysismembraneandinprocess