生物分离工程-双语版-2讲课稿.ppt

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1、生物分离工程-双语版-21IntroductionProteinfolding(1)shortrangedinteraction(secondarystructure)(2)gradualcombinationandreshuffling(重新组合）(subdomains,domains,andsubunitassemblies)Secondgenerationbiotechnologicalproducts(1)largermolecules(2)morecomplex(3)moreglycosylate(4)moresulphydrylb

2、onds1IntroductionInitialbioseparationimportance(1)filtration(2)centrifugation(3)chromatographyClassifiedheuristics（论据，规则）(1)unitoperationmethods(2)designstepsequence(3)species(componentsproperties)(4)composition1IntroductionThreefactorsofdesigningbioseparationprocesses(1)pu

3、rity(2)cost(3)marketMaterialstateandchoiceofseparationmethodsMaterialsecreted(ultrafiltration,centrifugation)Notsecretedmaterial(celldisruption,solid-liquidseparation)Materialinliquid(ultrafiltration,adsorption)Materialinsolid(extractionintoaqueoussolution)NewandwordsProtac

4、telawsuits诉讼Deleterious有害的，有毒的Vexing烦恼Hierarchic分等级Subdomain亚域Domains蛋白质结构的域Subunit亚单位Endoplasmicreticulum内质网Disulfidebond二硫键Academia学术界Delicate脆弱的Flowchart流程图Cytoplasmic细胞质的Periplasmic外周胞质Osmotic渗透urea脲Guanidine胍IIProductexcretionfromthecellorcelldisruptionExcretedproteina

5、dvantage(1)contaminationlevellow(2)easydownstreamprocessingsteps(3)notrequirecelldisruption(4)avoidhandlingviscousfluidsandproteinaserelease(5)facilityextractionofproducts(6)signalpeptidedeterminessecretionCelldisruption(1)energy-intensive(强烈）andviolent(2)withoutdestroyingi

6、nterestprotein(3)contain(兼容）process(bacteria,yeast,mammalian,plant,andinsectsources)(4)becontrolled(5)bevalidatedProductsrequiringcelldisruptionVaccines(tetanus(破伤风）,meningitis（脑膜炎)Glucokinase（葡萄糖激酶）Glycerokinase（甘油激酶）Invertase（转化酶）Toxin(enterotoxin)Subcellularcomponents(mi

7、tochondrai,chloroplast)Intracellularconstituents(DNA,RNApreparation,andvirus-likeparticles)RecombinantinsulinRecombinantgrowthhormoneProteinAandG.Ideallargescalecelldisrupter(1)disrupttoughorganisms(2)mechanismofcelldisruptionshouldbewellunderstood(3)sterilizable(4)containa

8、ble（兼容）andvalidatable(5)amenabletobeingmadeautomatic(6)continuousandcompact（紧凑的）(7