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1、世界华人消化杂志2009年3月8日;17(7):724-728ISSN1009-3079CN14-1260/R临床经验CLINICALPRACTICEDNA倍体分析与癌胚抗原检测对良、恶性腹水鉴别诊断的意义张爱军,许琳,李文利,马师洋,钮自宇,王青张爱军,许琳,李文利,马师洋,钮自宇,青岛市市立医院消化科山东省青岛市266011王青,青岛市市立医院东区消化科山东省青岛市266071作者贡献分布:此课题由张爱军,许琳及王青设计;研究过程由张爱军,许琳,李文利,马师洋及钮自宇操作完成;研究所用新试剂及分析工具由张爱军与王
2、青提供;数据分析由张爱军,许琳及李文利完成;本论文写作由张爱军完成.通讯作者:王青,266071,山东省青岛市东海中路5号,青岛市市立医院东区消化科.qingwangqingdao@yahoo.com.cn电话:0532-88905630收稿日期:2008-12-25修回日期:2009-01-25接受日期:2009-02-09在线出版日期:2009-03-08SignificanceofDNAploidyanalysisandcarcinoembryonicantigenassayfordifferentialdia
3、gnosisbetweenbenignandmalignantascitesAi-JunZhang,LinXu,Wen-LiLi,Shi-YangMa,Zi-YuNiu,QingWangAi-JunZhang,LinXu,Wen-LiLi,Shi-YangMa,Zi-YuNiu,DepartmentofGastroenterology,QingdaoMunicipalHospital,Qingdao266011,ShandongProvince,ChinaQingWang,DepartmentofGastroente
4、rology,QingdaoMunicipalHospitalEastBranch,Qingdao266071,ShandongProvince,ChinaCorrespondenceto:QingWang,DepartmentofGastroenterology,QingdaoMunicipalHospitalEastBranch,5DonghaiMiddleRoad,Qingdao266071,ShandongProvince,China.qingwangqingdao@yahoo.com.cnReceived:
5、2008-12-25Revised:2009-01-25Accepted:2009-02-09Publishedonline:2009-03-08AbstractAIM:ToinvestigatethedifferentialdiagnosticsignificanceofDNAploidyanalysisandserum,asceticcarcinoembryonicantigen(CEA),ascites/serumCEAratiosassayinmalignant(PHCandnon-PHCmalignancy
6、)andbenignascites.METHODS:Flowcytometry(FCM)wasusedforDNAploidyanalysisandenzymelinkedimmunosorbentassay(ELISA)forCEAassayin56malignant(31PHCand25non-PHC)ascites.Asacontrol,50nonmalignantasciteswereevaluatedatthesametime.IftheserumorasciticCEAvalue>5μg/L,andthe
7、ascites/serumCEAratios>1,thentheCEAassaywasdefinedaspositive.Ifananeuploidywasdetected,theresultoftheDNAploidyanalysiswasdefinedaspositive.RESULTS:ThesensitivityandspecificityofasciticCEA,ascites/serumCEAratios>1andaneuploidyindifferentialdiagnosisofPHCascitesa
8、ndnon-PHCwithmalignantascitesorwithbenignasciteswere35.48%,84%and72%,84%;35.48%,98%and72%,98%;70.97%,86%and71.43%,86%,respectively.Thesensitivityandspecificityofthecombinati
