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1、LactobacillusplantarumDK119asaProbioticConfersProtectionagainstInfluenzaVirusbyModulatingInnateImmunity莫小贝2013/11/29Min-KyungPark,VuNGO,Young-ManKwon,Young-TaeLee,Young-HeeCho,Sung-MoonHong,HyeSukHwang,Eun-JuKo,Yu-JinJungSieunYoo植物乳杆菌DK119通过调节先天免疫作为一种抗流感病毒的益生菌赋予保护Conte
2、ntAbstractIntroductionResultMaterialsandmethodsSummaryInnovationIntroductionInfluenzaisaseriousrespiratorydisease,currentvaccinesareonlyeffective,Antiviralmedicationsalsohavelimitations.Thus,itisdesirabletofindamoregeneralmeasurethatwouldhaveprotectiveeffectsoninfluenz
3、avirus.IntroductionProbioticsaremicro-organismswhichbestowhealthbenefitsonthehostwhenadministeredinadequateamounts.Themostcommonprobioticsincludelacticacidbacteria.Variousfermentedvegetablesanddairyproductscontainavarietyoflacticacidbacteriathatwereshowntoprovidehealth
4、benefitsandimprovementstoacertainlevelInnovationInpreviousstudies,theeffectsoflacticacidbacteriaoninfluenzaincludepartialsurvivalprotectionorprolongedsurvivalperiods.Itremainsunknownwhethertreatmentswithlacticacidbacteriacanconferimprovedprotectionbyreducingweightlosso
5、finfectedanimalsandthusamelioratingmorbidity.AbstractInthepresentstudy,wedeterminedwhethertheLactobacillusplantarumDK119(DK119)newlyisolatedfromthefermentedvegetablefood“Kimchi”wouldconferenhancedprotectionagainstthesubsequentinfluenzavirusinfectionviatheintranasaloror
6、aladministration.TheantiviralefficacyofDK119wasevaluatedbymonitoringweightchanges,andexamininglungviralloadsandhistology.MaterialsandmethodsPreparationof Lactobacillusplantarum DK119andVirusVirus:甲型H1N1流感A/PR8TreatmentofMicewithDK119andInfluenzaVirusA/PR8Materialsandme
7、thodsFemaleBALB/cmiceDK119(109,108,or107CFUpermouse)A/PR8H1N1virus(2.5LD50)MaterialsandmethodsLungViralTitersandCytokineAssaysMaterialsandmethodsTreatmentofMicewithClodronate-liposomesandFlowCytometryMaterialsandmethodsHistologyLungsampleswerecollectedafterbloodtakenvi
8、atheabdominalaorta,fixedin10%neutralbufferedformalinfor48hrs,transferredto70%ethanol,andembeddedinparaffin.Finally,se
