五子衍宗方对D-半乳糖致衰老小鼠学习记忆及抗氧化损伤的影响.pdf

《五子衍宗方对D-半乳糖致衰老小鼠学习记忆及抗氧化损伤的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、2012年10月第18卷第10期中国中医基础医学杂志October2012Vo1．18．No．10ChineseJournalofBasicMedicineinTraditiona1ChineseMedicine1093【实验研究】五子衍宗方对D一半乳糖致衰老小鼠学习记忆及抗氧化损伤的影响亲王光勇，狄国杰，曾晓，刘苗苗，何毓敏，袁丁，张长城，王婷△(1．宜昌市夷陵区人民医院，湖北宜昌443100；2．三峡大学医学院，湖北宜昌443000)摘要：目的：探讨五子衍宗方对Dgal致衰老小鼠学习记忆及抗氧化损伤的影响。方法：小鼠随机分为4组，模型组及给药组皮下注射D．gal，正常组皮下注射NaC1；

2、同时各组给予相应的药物治疗，8周后检测小鼠的学习记忆能力、小鼠的脑、脾脏及胸腺指数、血清中SOD和MDA水平。结果：五子衍宗方能延长小鼠目标象限停留时间，增加穿过平台次数，降低跳台错误次数；降低脑及胸腺重量指数，增加SOD活性，降低MDA含量。结论：五子衍宗方能改善衰老小鼠的学习记-I己能力。其机制可能与提高小鼠的抗氧化能力有关。关键词：五子衍宗方；D一半乳糖；衰老；学习记忆；抗氧化中图分类号：R285．5文献标识码：B文章编号：1006-3250(2012)101093-03InfluenceofWuZiYANZongprescriptiononD-galactoseinducedagin

3、gmicelearning．memoryandAnti0xidatiVedamageWANGGuang—yong，D1Guo—jie，ZENGXiao，LIUMiao—miao，HEYu—min，YUANDing，ZHANGChang—cheng，WANGTing(1．YichangYilingHospital，Yichang，Hubei，443100，China；2．MedicalCollegeofChinaThreeGrogesUniversity，Yichang，Hubei，443002，China)Abstract：0bjective：Thepresentstudywastoinve

4、stigatethelearningmemoryandanti—oxidativeeffectsofwuziYanzongFangonD—galactose—inducedsenescence．Methods：Eight-week—oldmalemiceweredividedintofourgroupsofvehicle—treatedgroup，D—galactose—treatedgroup，WuziYanzongFang(0．49g／kg、0．98g／kg)groups．MicewereinjectedwithD·galactose(150mg／kg／d)subcutaneouslya

5、ndintragastricallyadministeredWuziYanzongFangsimuhaneously．After8weeksoftreatment，theanimalbehaviorandjumpingstandweretested．Cerebralindex，spleenindexandthymusindexweremeasured．TheSODactivityandMDAcontentwereevaluatedbybiochemistrydetection．Results：WuziYanzongFangmarkedlyextendedthetimeofstayingint

6、hetargetquadrantandincreasedthenumberoftimesthroughtheplatform．ThenumberoferrorsinjumpingstandexperimentdecreasedsignificantlycomparedwithD-gal—treatedgroup．Meanwhile，WuziYanzongFangalleviatedtheatrophyofbrainandthymusinmice，elevatedactivityofserumsuperoxidedismutase，anddecreasedmalondia1dehydecont

7、ent．Conclusion：WuziYanzongFangcanimprovelearningandmemoryofagingmiceinducedbyD-galanditsmechanism，atleastinpart，viamodificationoftheredoxsystemintheorganism．Keywords：WuziYanzongFang；D—galactose；senescence；l