榄香烯注射液联合经皮瘤内注射无水乙醇治疗兔移植性VX2肝肿瘤的研究.pdf

《榄香烯注射液联合经皮瘤内注射无水乙醇治疗兔移植性VX2肝肿瘤的研究.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、医学影像学杂志2014年第24卷第8期JMedImagng。1．：实验研究●榄香烯注射液联合经皮瘤内注射无水乙醇治疗兔移植性VX2肝肿瘤的研究陈翼，王玎玲，何重阳(重庆市红十字会医院江北区人民医院放射科重庆400020)【摘要】目的探讨超声引导下经皮瘤内注射无水乙醇(percutaneousalcoholinjectiontherapy，PEIT)联合榄香烯注射液静脉给药治疗兔移植性VX2肝肿瘤的疗效及作用机理。方法建立兔移植性VX2肝肿瘤模型3O只，随机分为2组：经皮瘤内注射无水乙醇联合榄香烯注射液静脉滴注治疗组(15只

2、)；单纯经皮无水乙醇注射治疗组(15只)。实验动物在接种15天后每隔3天治疗一次，5次后处死。手术前、后抽兔耳缘静脉血行血生化检查，MSCT扫描测量肿瘤大小变化，同时进行组织细胞学与细胞超微结构的观察。结果PEIT+榄香烯注射液治疗组肿瘤增长率与坏死率与单纯PEIT治疗组比较差异均有统计学意义(Pd0．05)；TUNEL染色法检测显示PEIT~榄香烯注射液治疗组肿瘤细胞凋亡指数明显高于单纯PElT治疗组(PdO．05)；PEIT+榄香烯注射液治疗组血管内皮生长因子(VEGF)表达水平明显低于单纯PEIT治疗组(Pd0．05

3、)；前者2例出现肝内转移灶，而后者5例出现转移灶，差异性有显著意义(P<0．05)；治疗前后两治疗组血生化及肝‘肾功检查均差异无显著性意义(P>0．05)。结论PEIT联合榄香烯注射液治疗兔移植性VX2肝肿瘤可明显诱导肿瘤细胞凋亡、抑制VEGF的表达以及肝内转移。【关键词】兔；肝脏；VX2；模型；PElT；榄香烯注射液中图分类号：R361；R445．2文献标识码：A文章编号：1006—9011(2014)08—1376-04AnexperimentalstudyfortheimplantedVX2livertumorcel

4、lsinrabbitswithPEITcombinedwithelemeneinjectionCHENYi，W_ANGDing—ling．HEChong—yangDepartmentofRadiologyChongqingMunicipalCross—redHospitalTiangbeiDistrictPeoplesHospital，Chongqing400020，P．R．China[Abstract]ObjectiveToexploretheprinciple，effectandmethodofPEITcombined

5、withelemeneinjectionfortumorablation．MethodsThirtynewzealandwhiterabbitswithimplantedliverVX2tumorswererandomlydividedintotwogroups：PEIT+elemeneinjectiongroup(n一15)，percutaneousethanolinjection(PEI)group(n一15)．Fiftydaysaftertransplantation，thedrugswereinjectedinto

6、tumorsofalltherabbitsonceevery3days．After5timesofinjection，therabbitswerekilled．Thetumorsweremeasured3dimensionallywithCT。whiletheratesoftumornecrosis．thevolumeoftumorandthegrowthrateofthetumorwerecalculated．Liverandkidneyfunctiontests(AST，AIT，ALPandBUN)aswellaswh

7、itebloodcellcountswereassessedbeforeandaftertreatment．andCTexaminationwasperformedatthesametime．Themorphologicalandtheultramicrostructuralchangeswerealsoinvestigatedunderlightmicroscopeorelectronmi—croscope．ApoptoticcellswereevaluatedwithTUNELessay，inthesametime。v

8、ascularendothelialgrowthfactor(VEGF)andexpressionwereexaminedbyimmunohistochemistry．ResultsAmongall2groups。tumorgrowingrateforPElT+elemeneinjectiongroup