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时间:2020-05-23
《经皮瘤内注射艾迪注射液对兔移植性VX2肝肿瘤VEGF表达的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、医学影像学杂志2014年第24卷第5期JMedImagingVo1.24No.52014实验研究经皮瘤内注射艾迪注射液对兔移植性VX2肝肿瘤VEGF表达的影响陈翼,王玎玲,何重阳(重庆市红十字会医院江北区人民医院放射科重庆400020)【摘要】目的探讨超声引导下复方中药艾迪注射液瘤内注射治疗兔移植性VX2肝肿瘤的作用效果及机制。方法建立新西兰大白兔肝VX2移植性肿瘤模型28只,随机分为3组,即艾迪注射液局部注射治疗组(12只)、经皮无水乙醇注射(percutaneousethanolinjection,PEI)组(8只)、生理盐水局部注射治疗组(8只)。全部动物在接种2周后每隔3天向肿瘤内注入
2、治疗药物,4次后处死。于术前术后兔耳缘静脉抽血行血生化检查,同时行64排螺旋CT扫描测量并计算肿瘤体积、肿瘤增长率及肿瘤坏死率,以及进行免疫学、组织细胞学与细胞超微结构的观察。结果各实验组病灶均进行性增大,但艾迪注射液治疗组肿瘤增长率及坏死率最小,与生理盐水组比较差异均有统计学意义(P3、有4例和5例出现转移灶,有显著差异性(P0.05)。结论艾迪注射液能明显抑制兔肝移植性VX2肿瘤VEGF蛋白的表达。【关键词】兔;肝脏;VX2;模型,动物;艾迪注射液中图分类号:R735.7;R-332文献标识码:A文章编号:1006—9011(2014)05—0859-06ExpressionofVEGFintheimplantedVX2livertumorcellsinrabbitswithpercutaneousintratumorAiDiinjectionCHE4、NYi,WANGDing—ling,HEChong—yangDepartmentofRadiology,TheFirstPeople'sHospitalofJiangbei,Chongqing400020,P.R.China[Abstract]ObjectiveToexploretheprinciple,effectandmethodofpercutaneousAiDiinjectionfortumorablation.MethodsTwenty-eightnewzealandwhiterabbitswithimplantedliverVX2tumorswererandomlydividedi5、ntothreegroups:AiDiinjectiongroup(n一12),percutaneousethanolinjection(PEI)group(n一8),andphysiologicalsalinegroup(n一8).Threeweeksaftertransplantation,thedrugswereinjectedintotumorsofalltherabbitsonceevery3days.After4timesinjection,therabbitswerekilled.Thetumorsweremeasured3dimensionallywithCT,whilethe6、ratesoftumornecrosis。thevolumeoftumorandthegrowthrateofthetumorwerecalculated.Liverandkidneyfunctiontests(AST,ALT,ALPandBUN)aswel1aswhitebloodcellcountswereassessedbeforeandaftertreatment,andCTexaminationwasperformedatthesametime.Themorphologicalandtheultramicrostructuralchangeswerealsoinvestigatedu7、nderlightmi—croscopeorelectronmicroscope.Vascularendothelialgrowthfactor(VEGF)andexpressionwereexaminedbyimmuno—histochemistry.ResultsTumorsinallgroupskeptgrowingaftertheprocedure.Amongall3groups,tumo
3、有4例和5例出现转移灶,有显著差异性(P0.05)。结论艾迪注射液能明显抑制兔肝移植性VX2肿瘤VEGF蛋白的表达。【关键词】兔;肝脏;VX2;模型,动物;艾迪注射液中图分类号:R735.7;R-332文献标识码:A文章编号:1006—9011(2014)05—0859-06ExpressionofVEGFintheimplantedVX2livertumorcellsinrabbitswithpercutaneousintratumorAiDiinjectionCHE
4、NYi,WANGDing—ling,HEChong—yangDepartmentofRadiology,TheFirstPeople'sHospitalofJiangbei,Chongqing400020,P.R.China[Abstract]ObjectiveToexploretheprinciple,effectandmethodofpercutaneousAiDiinjectionfortumorablation.MethodsTwenty-eightnewzealandwhiterabbitswithimplantedliverVX2tumorswererandomlydividedi
5、ntothreegroups:AiDiinjectiongroup(n一12),percutaneousethanolinjection(PEI)group(n一8),andphysiologicalsalinegroup(n一8).Threeweeksaftertransplantation,thedrugswereinjectedintotumorsofalltherabbitsonceevery3days.After4timesinjection,therabbitswerekilled.Thetumorsweremeasured3dimensionallywithCT,whilethe
6、ratesoftumornecrosis。thevolumeoftumorandthegrowthrateofthetumorwerecalculated.Liverandkidneyfunctiontests(AST,ALT,ALPandBUN)aswel1aswhitebloodcellcountswereassessedbeforeandaftertreatment,andCTexaminationwasperformedatthesametime.Themorphologicalandtheultramicrostructuralchangeswerealsoinvestigatedu
7、nderlightmi—croscopeorelectronmicroscope.Vascularendothelialgrowthfactor(VEGF)andexpressionwereexaminedbyimmuno—histochemistry.ResultsTumorsinallgroupskeptgrowingaftertheprocedure.Amongall3groups,tumo
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