柑橘溃疡病生防细菌的分离鉴定-论文.pdf

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1、热带作物学报2014，35(7)：1398—1403ChineseJournalofTropicalCrops柑橘溃疡病生防细菌的分离鉴定陈娇梅，蔡学清1，邱思鑫：，胡方平1福建农林大学植物保护学院．福建福州3500022福建省农业科学院作物研究所．福建福州350013摘要为了从柑橘上获得能防治柑橘溃疡病的生防细菌．从福州3个地区的柑橘园采集不同柑橘品种的叶片、春稍和花．用稀释分离法分离得到84株细菌菌株．用平板抑菌圈法筛选获得ll株对柑橘溃疡病菌具有拮抗作用的菌株；用离体叶片防效筛选获得39株对柑橘溃疡病具有50％以上防效的菌株．占菌

2、株总数的46．4％。对3株防效显著的拮抗菌株YH1、YS5和NY20进一步进行防效测定，结果表明：菌株培养液的防治效果最好，菌体次之，代谢产物最差；对这3株细菌的8—1，4一葡聚糖酶、蛋白酶和B一1，3—1，4-葡聚糖酶的酶活性进行测定，结果表明酶活性的强弱与防病效果有一定的相关性。经过16SrDNA序列分析，革兰氏染色、鞭毛染色、芽孢染色和生理生化测定，确定这3个菌株均属于芽孢杆菌属，其中菌株YH1鉴定为短小芽孢杆菌Bac驸口“m砌。关键词柑橘溃疡病；拮抗菌；芽孢杆菌；生物防治中图分类号$476文献标识码AIsolationandId

3、entificati0nofBiocontrolBacteriafromCitrustoXanthomonasaxonopodispV．citriCHENJiaomei，CAIXueqing件，QIuSixin，HUFangping1CollegeofPlantProtection,~qianAgricultureandForestryUniversity,Fuzhou,~ujian350002，China,"2InstituteofCropSciences，F~jianAcademyofAgriculturalSciences，Fuz

4、hou,Fujian350013，ChinaAbstractToobtainbiocontrolbacteriafromcitruswhichcouldcontrolcitruscanker,84bacterialstrainswereisolatedfromleaves，springshootsandflowe~ofdifferentcitrusvarietiescollectedfromthreecitrusorchardsinFuzhou．Amongthem，elevenisolateswhichcouldinhibitthegr

5、owthofXanthomonasaxonopod~pv．citri(Xac．)werescreenedoutbytheinhibitingzonemethod．ButwhenallisolateswereusedtocontrolthepathogenOncitrusleavesinvitro，39bacterialisolateshad46．4％controleficacy．Furthertestsshowedthat3strains．YH1．YS5andNY20had90．9％一100％controlefficaciesagain

6、stXac．after14daysofinoculation．TheNBcultures．cellsandmetabolitesofthe3strainsweretestedinvitrotocontrolthecitruscankeronleaves．Theresultsshowedthatthediseasecouldbecontrolledbyal1treatments．Buttheculturesofthebacteriahadthebestcontrolefectandthemetabolitesweretheworst．An

7、alyzingactivitiesofthe13-1，4一endoucanase，proteaseandp一1，3—1，4一end0g1ucanaseofthe3strainsshowedthattheenzymeactivitieshadsomecertaincorrelationwithdiseasecontrolefect．Basedontheresultsof16SrDNAsequencesanalysis，gram，flagellaandsporestains，physiologicalandbiochemicaltests，

8、the3strainswereidentifiedasmembersofBacillus，andthestrainYH1as且pumilus．KeywordsXanthomonasaxonopodiapv．