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1、中国农业科学2007,40(5):932-939ScientiaAgriculturaSinica中国野生柑橘上衰退病毒分离株分子特征分析11,2212易龙，周常勇，周彦，王志刚，唐科志（1西南大学植物保护学院，重庆400716；2中国农业科学院柑橘研究所，重庆400712）摘要：【目的】了解中国野生柑橘上携带柑橘衰退病毒（CTV）分离株的CP/HinfⅠRFLP组群构成和分子特征。【方法】运用RT-PCR对采自中国云南、广西、四川、湖南、江西等省11个野生柑橘上CTV分离株的病毒衣壳蛋白基因(CPG)进行扩增，利用RFLP和SSCP对CPG进行分析，并将测定的CP
2、G序列进行同源性比较和聚类分析。【结果】发现野生柑橘上11个CTV分离株以单一组群感染为主；这些CTV分离株CPG的核苷酸序列和相应的氨基酸序列同源性分别为92.5％～99.5％和95.0％～100％；与GenBank收录的9个全基因组CTV分离株的相应基因序列进行聚类分析，其核苷酸序列和氨基酸序列同源性分别为92.1％～98.8％和94.6％～100％。【结论】序列同源性比较说明CTVCPG具有高度保守性，同时系统进化分析表明收集到的野生CTV分离株与国外分离株分属5大类群，具有较复杂的亲缘关系。关键词：野生柑橘；柑橘衰退病毒；多态性分析；序列比对；系统进化Mol
3、ecularCharacterizationofCitrusTristezaVirusIsolatesinChineseWildTypeCitrusYILong2,ZHOUChang-yong1,2,ZHOUYan1,WANGZhi-gang2,TANGKe-zhi1(1CollegeofPlantProtection,SouthwestUniversity,Chongqing400716;2CitrusResearchInstitute,ChineseAcademyofAgriculturalSciences,Chongqing400712)Abstract:【O
4、bjective】ThisstudywasconductedtoinvestigatetheCP/HinfⅠRFLPgroupsandmolecularcharacterizationofcitrustristezavirus(CTV)isolatesfoundinwildChinesecitrusplants.【Method】Thetargetsequencesofcoatprotectiongene(CPG)wereamplifiedandsequencedfrom11CTVisolatesextractedfromthesamplesofwildcitrusp
5、lantscollectedinYunnan,Guangxi,Sichuan,HunanandJiangxiprovinces,usingRT-PCRandsequencer.CPGsoftheseisolateswereanalyzedbyRFLPandSSCP,andtheCPGsequencesofthe11Chineseisolatesand9foreignisolatesfromGenBankweresubjectedtosequenceanalysisandcomparedbetweeneachbyBioEidtandMEGAsoftware.【Resu
6、lt】CPGnucleotidesequencesofthe11ChineseCTVisolatesand9foreignisolateswerefoundtohaveidentityrangingfrom92.5%to99.5%and95.0%to100%,whereasidentityoftheiraminoacidsequencesrangesfrom92.1%to98.8%and94.6%to100%,respectively.RFLPandSSCPanalysisonCPGof11CTVisolatesdemonstratedthatsingleCP/Hi
7、nfⅠRFLPgroupswerefoundinmostwildtypecitrus.【Conclusion】Phylogeneticanalysissuggeststhatthe11ChineseCTVisolatesand9foreignCTVisolatesbelongtofivegroups,CPGwashighlyconserved.ItcanbeconcludedthatthegeneticrelationshipbetweenCTVisolatesiscomplicated.Keywords:Wildcitrus;Citrustristezavir
