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1、.水稻小粒密穗突变体sep1的遗传分析、基因定位与育种利用王跃星1,# 吴昆2,# 方云霞1 红旗1 倪深1 付向东2 朱旭东1,∗(1中国水稻研究所水稻生物学国家重点实验室,310006;2中国科学院遗传与发育生物学研究所,100101;#共同第一作者;∗通讯联系人,EGmail:ricezxd126.)GeneticAnalysis,GeneMappingandBreedingApplicationofSmallGrainandDensePanicleMutantsep1inRiceWANG
2、YueGxing1,#,WUKun2,#,FANGYunGxia1,CHENHongGqi1,NIShen1,FUXiangGdong2,ZHUXuGdong1,∗(1StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou310006,China;2InstituteofGeneticsandDevelopingBiology,ChineseAcademyofSciences,Beijing10010
3、1,China;#Theseauthorscontributedequallytothispaper;∗Correspondingauthor,EGmail:ricezxd126.)WANGYuexing,WUKun,FANGYunxia,etal.Geneticanalysis,genemappingandbreedingapplicationofsmallgrainanddensepaniclemutantsep1inrice.ChinJRiceSci,2014,28(3):229G234.
4、Abstract:By60CorGrayradiation,asmallgrainanddensepaniclemutantsep1wasobtainedfromtheindicavarietyShuangkezao.ComparedtothewildtypeShuangkezao,itwasamutantwithsmallergrain,shorterspikeGstalkbutmoregrainnumberperpanicle.Thehistologicalsliceobservationo
5、fthemainstemshowedthatthemutanthadmorevascularbundlenumberinonecycleandmorecellnumberperunitareathanthewildtype.Geneticandallelismanalysisresultsshowedthatthesmallgrainanddensepaniclemutantwascontrolledbyarecessivegene,anditwasdifferenttothedensepani
6、clegeneswhichhadbeenalreadycloned.ThenbyconstructingthegeneticsegregatedpopulationandusingtheInDelmarkers,thesmallgrainanddensepaniclegenewasmappedonthecentromericregionofchromosome5betweenmarkerXP26G28andXP26G31.Inaddition,thebreedingapplicationofth
7、emutantwascarriedoutonCMSlinesandthestabilelinesofhighgenerationshadfinallybeenobtained.Keywords:rice;smallgrainanddensepanicle;geneticanalysis;genemapping;breedingapplication王跃星,吴昆,方云霞,等.水稻小粒密穗突变体sep1的遗传分析、基因定位与育种利用.中国水稻科学,2014,28(3):229G234.摘 要:通过6
8、0Co衰变产生的r射线辐照籼稻品种“双科早”,获得小粒密穗突变体sep1,该突变体与野生型双科早相比表现为籽粒变小,穗轴变短,着粒密度增加.主茎切片观察结果显示,突变体维管束数和单位面积细胞数均比野生型多.遗传分析和等位性分析结果表明,该突变体为隐性突变,与已克隆的直立密穗基因不等位.利用InDel分子标记将小粒密穗基因sep1定位在第5染色体着丝粒附近的两个标记XP26G28与XP26G31之间,物理距离约为3.896Mb.此外,还利用该突变体开展了相应的育种利用研究,已获得高代稳定的不育系材