资源描述:
《水稻小粒密穗突变体sep1的遗传分析、基因定位与育种利用》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、水稻小粒密穗突变体sep1的遗传分析、基因定位与育种利用王跃星1,# 吴昆2,# 方云霞1 陈红旗1 倪深1 付向东2 朱旭东1,∗(1中国水稻研究所水稻生物学国家重点实验室,杭州310006;2中国科学院遗传与发育生物学研究所,北京100101;#共同第一作者;∗通讯联系人,EGmail:ricezxd@126.com)GeneticAnalysis,GeneMappingandBreedingApplicationofSmallGrainandDensePanicleMutantsep1inRiceWANGYueGxing1,#,WUKun2,#,FANGY
2、unGxia1,CHENHongGqi1,NIShen1,FUXiangGdong2,ZHUXuGdong1,∗(1StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou310006,China;2InstituteofGeneticsandDevelopingBiology,ChineseAcademyofSciences,Beijing100101,China;#Theseauthorscontributedequallytothispaper;∗Correspo
3、ndingauthor,EGmail:ricezxd@126.com)WANGYuexing,WUKun,FANGYunxia,etal.Geneticanalysis,genemappingandbreedingapplicationofsmallgrainanddensepaniclemutantsep1inrice.ChinJRiceSci,2014,28(3):229G234.Abstract:By60CorGrayradiation,asmallgrainanddensepaniclemutantsep1wasobtainedfromtheindica
4、varietyShuangkezao.ComparedtothewildtypeShuangkezao,itwasamutantwithsmallergrain,shorterspikeGstalkbutmoregrainnumberperpanicle.Thehistologicalsliceobservationofthemainstemshowedthatthemutanthadmorevascularbundlenumberinonecycleandmorecellnumberperunitareathanthewildtype.Geneticandal
5、lelismanalysisresultsshowedthatthesmallgrainanddensepaniclemutantwascontrolledbyarecessivegene,anditwasdifferenttothedensepaniclegeneswhichhadbeenalreadycloned.ThenbyconstructingthegeneticsegregatedpopulationandusingtheInDelmarkers,thesmallgrainanddensepaniclegenewasmappedonthecentro
6、mericregionofchromosome5betweenmarkerXP26G28andXP26G31.Inaddition,thebreedingapplicationofthemutantwascarriedoutonCMSlinesandthestabilelinesofhighgenerationshadfinallybeenobtained.Keywords:rice;smallgrainanddensepanicle;geneticanalysis;genemapping;breedingapplication王跃星,吴昆,方云霞,等.水稻小粒
7、密穗突变体sep1的遗传分析、基因定位与育种利用.中国水稻科学,2014,28(3):229G234.摘 要:通过60Co衰变产生的r射线辐照籼稻品种“双科早”,获得小粒密穗突变体sep1,该突变体与野生型双科早相比表现为籽粒变小,穗轴变短,着粒密度增加.主茎切片观察结果显示,突变体维管束数和单位面积细胞数均比野生型多.遗传分析和等位性分析结果表明,该突变体为隐性突变,与已克隆的直立密穗基因不等位.利用InDel分子标记将小粒密穗基因sep1定位在第5染色体着丝粒附近的两个标记XP26G28与XP26G31之间,物理距离约为3.896Mb.此外,还利用该突变体开
8、展了相应的育种利用研究,