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ID:56432601
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时间:2020-06-18
《培养基中的矿质元素.ppt》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、NutrientMediaforPlantTissueCultures刘 彤石河子大学生命科学学院1刘彤 石河子大学生命科学学院Oneofthefirstdecisionsthatmustbemadewhendevelopingatissueculturesystemiswhatmediumtouse.Nutrientmediaforplanttissueculturearedesignedtoallowplanttissuestobemaintainedinatotallyartificialenvironment.2刘彤 石河子大学生命科学学院F
2、unctionsofmediumProvidewaterProvidemineralnutritionalneedsProvidevitaminsProvidegrowthregulatorsAccesstoatmosphereforgasexchangeRemovalofplantmetabolitewaste3刘彤 石河子大学生命科学学院A.MacroelementsconsistofN,K,P,Ca,Mg,andS.1.Nitrogen(N)-Nitrogenisessentialforplantgrowth.Mostinorganicnitr
3、ogenisconvertedtoaminoacidsandthentoproteins.Nitrogenistypicallyaddedtoplantnutrientmediaasthenitrateion(NO3-,oxidized)and/ortheammoniumion(NH4+,reduced),whichareaddedasinorganicsalts.Inorganicnitrogengenerallyrangesfrom25-60mMinnutrientmedia.Indevisingmedia,boththetotalamounto
4、fnitrogenaswellastherelativeamountsofNO3-andNH4+areimportant.ThereareusuallylowerlevelsofNH4+thanNO3-inmedium;nitrateisusuallyaddedatconcentrationsbetween25and20mMandammoniumatconcentrationsbetween2and20mM.42.Potassium(K)-Potassiumisthemajorioninplantswithapositivecharge,balanc
5、ingnegativeions.Althoughtheamountofpotassiumrequiredvarieswidelyamongdifferentspecies,inmediapotassiumconcentrationisgenerallycorrelatedwiththatofnitrateandrangesbetween20-30mM.5刘彤 石河子大学生命科学学院3.Phosphorous(P)-Phosphorusisanintegralpartofnucleicacidsandotherstructuralcompounds.I
6、tisaddedtoculturemediumasphosphate(PO4-)insodiumorpotassiumhydrogenphosphatesinconcentrationsrangingfrom1-3mM.4.Calcium(Ca)-Calciumisaco-factorofmanyenzymesandisparticularlyimportantincellwallsynthesis.Itissuppliedmostlyascalciumchlorideorcalciumnitrate,concentrationsrangingbet
7、ween1and3mM.Inplantcultures,calciumdeficiencymayresultinshoottipnecrosis.6刘彤 石河子大学生命科学学院5.Magnesium(Mg)-Magnesiumiscriticalforthefunctioningofenzymes,isanintegralcomponentofthechlorophyllmolecule,andisacationthatbalancesnegativeions.Itisusuallyaddedasmagnesiumsulfateinconcentra
8、tionssimilartothatofcalcium.6.Sulfur(S)-Sulfurisaparto
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