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1、Sporozoans孢子虫.Plasmodiumvivax(P.v)间日疟原虫.Plasmodiumfalciparum(P.f)恶性疟原虫.Toxoplasmagondii(T.gondii)刚地弓形虫.Cryptosporidium隐孢子虫.Sarcocystissuihominis猪人肉孢子虫.Blastocystishominis人芽囊原虫PurposeandRequirements.Mastermorphologicalcharacteristicsoferythrocyticcycleofplasmodiumv
2、ivax;.Mastermorphologicalcharacteristicsofearlytrophozoiteandgametocyteofplasmodiumfalciparum;.Familiarwithmorphologicalcharacteristicsofexoerythrocyticstageandthephasesinthemosquito;.Familiarwithpreparationofbloodfilmandstain;.Familiarwithmorphologicalcharacteris
3、ticsoftrophozoiteandcystofToxoplasma;1.ObservationofglassslideNo.原-5至No.原-8拿到玻片标本后请马上检查核对是否有漏、重复或破损，有问题的请马上举手报告老师！No.原-5:间日疟原虫,R,T,S,GP.vivax,R,T,S,GNo.原-6:恶性疟原虫,环状体P.falciparum,RingNo.原-7:恶性疟原虫,配子体P.falciparum,GametocyteNo.原-8:刚地弓形虫滋养体T.gondiitrophozoite注意：①在观察玻片
4、标本前，先将显微镜光线调到最强！②本次课只使用10倍镜和油镜；No.原-8:刚地弓形虫滋养体T.gondiitrophozoiteOvaltocrescent-shaped,and1nucleus1.ObservationofglassslideNo.原-5至No.原-8要求拍照No.原-7:恶性疟原虫,配子体falciparum,GametocyteGametocyte,GMaleG/Microgametocyte.Sausageshape腊肠形.ChromatinisdiffuseFemaleG/Macrogameto
5、cyte.Cescentshape新月形.Chromatiniscompact1.ObservationofglassslideNo.原-5至No.原-8要求拍照,不分雌雄Observation:glassslideNo.原-6to原-8No.原-6:恶性疟原虫,环状体P.falciparum,Ring.Chromatinisadot-like;.Cytoplasmisthedelicatering,blue-stained;.Occupyone-fifthofRBCindiameter.multipleinfection
6、早期滋养体/环状体Theearlytrophozoit/ringform，R要求拍照No.原-5:间日疟原虫,R,T,S,G1.ObservationofglassslideNo.原-5至No.原-8早期滋养体/环状体Theearlytrophozoit/ringform，R.核1个,点状.胞质环形,胞质与核之间有空泡,呈戒指状,.大小约为红细胞直径的1/3.被寄生红细胞无改变No.原-5:间日疟原虫,R,T,S,G1.ObservationofglassslideNo.原-5至No.原-8晚期滋养体/大滋养体thelar
7、getrophozoit,T被寄生红细胞体积稍增大,色淡,出现鲜红薛氏小点.核1个,.胞质增多变形,形状不规则，有空泡，.胞质内出现棕褐色烟丝状的疟色素.要求拍照之一No.原-5:间日疟原虫,R,T,S,G1.ObservationofglassslideNo.原-5至No.原-8未成熟裂殖体Immatureschizont,IMS.被寄生红细胞体积增大,色淡,出现鲜红薛氏小点..核分裂,数目<12个,.胞质渐变圆，空泡消失,.疟色素分散在胞质中.要求拍照之一No.原-5:间日疟原虫,R,T,S,G1.Observatio
8、nofglassslideNo.原-5至No.原-8成熟裂殖体Matureschizont,MS被寄生红细胞体积增大,色淡,出现鲜红薛氏小点.核分裂,数目>12个,.胞质分裂,围绕核,形成裂殖子,数目12-24个,.疟色素集中成1-2团，位于虫体中央或一侧.要求拍照之一No.原-5:间日疟原虫,R,T,
