生物信息学实习1 宋捷.doc

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1、生物信息学实习结课论文学院：生命学院专业班级：生技122姓名：宋捷学号：2012014069指导老师：庞红侠生物信息学实习一宋捷生技1222012014069作业：1.DothefollowingsearchwithEntrezatNCBI,describethestepsandanswerthequestions.Thenumberofnucleotidesequencerecordsassociatedwithbreastcancer在entre中搜索乳腺癌加引号，下滑找到核酸相关Numberoftheabovesequenc

2、erecordsthatarefromtheRefSeqdatabase在结果左面的菜单里显示相关数据库该词条相关数量3)NumberofnucleotidesequencesassociatedwithbreastcancerthataremRNAsmRNA(20,134)4)NumberoftheabovemRNAsequencerecordsthatareinRefSeqandthewordsbreastcancerappearsintheirtitles语法："breastcancer"[AllFields]AND(bio

3、mol_mrna[PROP]ANDrefseq[filter])5)NumberofHumanRefSeqmRNAsequencerecordswiththewordsbreastcancerintheirtitles语法：("human"[Organism])AND("breastcancer"[AllFields]ANDbiomol_mrna[PROP]ANDrefseq[filter])Results:1to20of36036)NumberofHumangeneBRCA1RefSeqmRNAsequencerecordswi

4、ththewordsbreastcancerintheirtitles语法：((("breastcancer"[AllFields]ANDbiomol_mrna[PROP]ANDrefseq[filter]))AND"brca1"[GeneName])AND"human"[Organism]Results:57)AccessionnumberofthemRNArecordforhumanBRCA1,transcriptvariant1,inRefSeq.Accession:NM_007294.3GI:2377572838)Tota

5、lnumberofnucleotidesreportedinthisrecord7,224bplinearmRNA9)ExactchromosomallocationofBRCA1,transcriptvariant1onthehumangenome/map="17q21"10)Numberoftimesthissequencewasupdated版本号从.1~.3应该是共有三次录入11)TotalnumberofaminoacidsthatthismRNAencodes数的….1863（word不含空格）点到ID号里有该蛋白质序

6、列的详细信息12)IdentifythelastaminoacidintheencodedproteinY是酪氨酸13)TotalnumberofExonsandIntronsinthisgenevariant(BRCA1,transcriptvariant1)Exon23个misc_feature26个网页搜索数14)Totalnumberofexons(partialorcomplete)inthe5’UTRofBRCA1,transcriptvariant15’非编码区，在CDS前有2个外显子15)Totalnumberof

7、exons(partialorcomplete)inthe3’UTRofBRCA1,transcriptvariant1CDS后，在其后有一个外显子16)BRCA1,transcriptvariant1encodesfulllengthBRCA1protein(isoform1).ProvidethenamesofothervariantsoftheBRCA1whichalsocodeforfulllengthproteins.AlsoprovidetheaccessionnumbersoftheirmRNAsequencesin

8、RefSeq.17)Inwhatway(s)istheBRCA1,transcriptvariant3mRNAdifferentthanBRCA1,transcriptvariant1?（hint:lookintothecommentsection