脑深部电刺激治疗帕金森病作用机制的探讨.pdf

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1、中国微侵袭神经外科杂志2012年O3月20日第l7卷第3期ChinJMinimInvasiveNeurosttrg，VOL．17，NO．3，March20，2012·107··临床研究·脑深部电刺激治疗帕金森病作用机制的探讨王尔松，季耀东，胡锦，王晨，姚慧斌，呼建文，江澄川【摘要】目的从蛋白组学的角度探索脑深部电刺激(DBS)与毁损术治疗帕金森病的机制是否类同。方法采用荧光差异凝胶电泳(DIGE)技术，测定3例行双侧丘脑底核DBS治疗的帕金森病病人脑脊液中蛋白，在手术前(未干预组)、手术7d后但未刺激前(微毁损组)和刺激1周后(DBS组)

2、的表达变化。另留取3例非中枢系统疾病病人的脑脊液蛋白作为正常对照组。结果未干预组和微毁损组、未干预组和DBS组、微毁损组和DBS组的组间比较分别发现14、18和13个明显差异蛋白点。除3个蛋白点外，未干预组和微毁损组与未干预组和DBS组的组间蛋白差异点完全不同；且在这3个相同的蛋白点中，两个蛋白点呈相反方向表达。结论结果初步提示DBS与毁损术治疗帕金森病的机制不相同。【关键词】帕金森病；脑深部电刺激；毁损术中图分类号：R742．5文献标志码：A文章编号：1009—122X(2012)03—01O7—03Studyofmechanismso

3、fdeepbrainstimulationforParkinson’SdiseaseWangErsong，JiYaodong，HuJin，WangChen，YaoHuibin，HuJianwen，JiangChengchuan。1．DepartmentofNeurosurgery，JinshanHospital，FudanUniversity，Shanghai200540，China；2．DepartmentofNeurosurgery，HuashanHospital，FudanUniversity，Shanghai200040，Chin

4、a；3．ResearchCenterforProteomeAnalysis，ShanghaiInstitutesforBiologicalSciences，ChineseAcademyofSciences，Shanghai200031，ChinaAbstract：ObjectiveTostudysimilaritiesanddiferencesinmechanismsbetweendeepbrainstimulation(DBS)andlesioningoperationforParkinson’Sdisease(PD)fromthepe

5、rspectiveofproteomics．MethodsTheproteinsinthecerebrospinalfluidswereassayedbeforeoperation(noninterventiongroup)，the7thdayafteroperationwithnostimulation(slightly-lesionedgroup)，and1weekafterstimulation(DBSgroup)respectively，in3patientswithPDwhohadundergonebilateralsubtha

6、lamicnucleusDBS．Andthecerebrospinalfluidproteinstakenfrom3patientswithnon-centralnervoussystemdiseasesservedasnormalcontrolgroup．Two-dimensionaldiferencegelelectrophoresis(DIGE)techniquewasusedtodeterminetheproteinchange．ResultsTherewere14，18，and13significantlyalteredprot

7、einspotsbetweennoninterventiongroupandslightly-lesionedgroup，noninterventiongroupandDBSgroup，slightly-lesionedgroupandDBSgrouprespectively．Withinthesealteredproteinspotsfromnonintervention／slightly—lesionedgroupsandnonintervention／DBSgroups，therewereonlythreeidenticalprot

8、einspotsinpairedgroups，ofwhichtwowereregulatedindiferentways．ConclusionsOurpreliminaryresultssug