欢迎来到天天文库
浏览记录
ID:55972780
大小:785.82 KB
页数:4页
时间:2020-06-03
《硫酸粘杆菌素体外抑菌效果的研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第34卷第2期郑州牧业工程高等专科学校学报Vo1.34No.22014年5月JournalofZhengzhouCollegeofAnimalHusbandryEngineeringMay2014硫酸粘杆菌素体外抑菌效果的研究李梦云,胡迎利。,乔宏兴,夏梦芳,郭建来。(1.河南牧业经济学院,河南郑州450011;2.郑州市饲料资源开发重点实验室,河南郑州450011)3.郑州市海润中慧饲料有限公司,河南郑州450056)摘要:将硫酸粘杆菌素按照在畜禽实际用量(2~2Omg/kg)选择3个浓度(5mg/kg、10mg/kg、20mg/k
2、g)分别配制溶液,按照常规方法制备成药敏片,分别置于接有大肠杆菌、沙门氏菌的普通琼脂板上,同时用标准药敏片庆大霉素、新霉素、强力霉素、盐酸环丙沙星、氧氟沙星、头孢曲松作对照,观察它们对大肠杆菌、沙门氏菌的抑菌效果。结果表明:①3个浓度的硫酸粘杆菌素对大肠杆菌和沙门氏菌均高度敏感,并随药物浓度的增加抑菌圈也相应增大。②硫酸粘杆菌素对大肠杆菌、沙门氏菌的抑菌效果显著优于庆大霉素、新霉素、强力霉素、盐酸环丙沙星、氧氟沙星、头孢曲松等抗生索(P3、章编号:1008—3111(2014)02—0008—03StudyonColistinsulfateantibacterialeffectinvitroLIMeng-yun,HUYing—li。,QIAOHong—xing,XIAMeng—fang。,GUOJian—lai。(1.HenanUniversityofAnimalHusbandryandEconomy,ZhengzhouHenan450011,China;2.KeyLaboratoryofFeedResourcesExploitation,ZhengzhouHenan44、50011,China;3.ZhengzhouHaiRunZhongHuiFeedCO.,LTD,ZhengzhouHenan450056,China)Abstract:Choosethreeconcentrations(5mg/kg,lOmg/kg,20mg/kg)ofColistinsulfatesolutions,basedonthedosageinlivestockandpoultry(2~20mg/kg).andpreparethreedrugsensitivepiecesandinoculatewithEscherichi5、aColiandsalmonellainordinaryAGARplate.Atthesametimeusingthestandarddrugsensitivepiecesofgentamicin,neo—mycin,doxycycline,ciprofloxacinhydrochloride,ofloxacinandceftriaxone,alsoinoculateinwithe.coli,salmonellainordinaryAGARplateasacontrolledtria1.Underthesameconditions,o6、bservetheirbacteriostaticeffectonescherichiacoliandsalmonella..Theresultsshowedthat①3concentrationsofColistinsulfateareallhighlysensitivetOe.coliandsalmonella,andhadbetterbacteriostaticeffectwiththeincreaseofdrugconcentration.②ThebacteriostaticeffectonE.coilandSalmonell7、aofColistinsulfatearesignificantlybetterthanthatofGentamicin,Neomycin,Doxycycline,Ciprofloxacin,Levofloxacinhydrochloride,andCeftriaxone(P<0.05).Keywords:colistinsulfate;escherichiacoli;salmonella;antibacterialeffect;invitro硫酸粘杆菌素是由多黏芽孢杆菌所产生的碱性的问题,从而给食品安全带来潜在的隐患。为了规抗革兰氏8、阴性菌的多肽抗生素,对大肠杆菌、沙门氏范用药,本试验选择3个用药剂量(5mg/kg、菌等有很好的杀菌效果n]。在猪和鸡上的研究表10mg/kg和20mg/kg)配制溶液,并用生产中常用明,日粮中添加硫酸粘杆菌素能显著提高
3、章编号:1008—3111(2014)02—0008—03StudyonColistinsulfateantibacterialeffectinvitroLIMeng-yun,HUYing—li。,QIAOHong—xing,XIAMeng—fang。,GUOJian—lai。(1.HenanUniversityofAnimalHusbandryandEconomy,ZhengzhouHenan450011,China;2.KeyLaboratoryofFeedResourcesExploitation,ZhengzhouHenan4
4、50011,China;3.ZhengzhouHaiRunZhongHuiFeedCO.,LTD,ZhengzhouHenan450056,China)Abstract:Choosethreeconcentrations(5mg/kg,lOmg/kg,20mg/kg)ofColistinsulfatesolutions,basedonthedosageinlivestockandpoultry(2~20mg/kg).andpreparethreedrugsensitivepiecesandinoculatewithEscherichi
5、aColiandsalmonellainordinaryAGARplate.Atthesametimeusingthestandarddrugsensitivepiecesofgentamicin,neo—mycin,doxycycline,ciprofloxacinhydrochloride,ofloxacinandceftriaxone,alsoinoculateinwithe.coli,salmonellainordinaryAGARplateasacontrolledtria1.Underthesameconditions,o
6、bservetheirbacteriostaticeffectonescherichiacoliandsalmonella..Theresultsshowedthat①3concentrationsofColistinsulfateareallhighlysensitivetOe.coliandsalmonella,andhadbetterbacteriostaticeffectwiththeincreaseofdrugconcentration.②ThebacteriostaticeffectonE.coilandSalmonell
7、aofColistinsulfatearesignificantlybetterthanthatofGentamicin,Neomycin,Doxycycline,Ciprofloxacin,Levofloxacinhydrochloride,andCeftriaxone(P<0.05).Keywords:colistinsulfate;escherichiacoli;salmonella;antibacterialeffect;invitro硫酸粘杆菌素是由多黏芽孢杆菌所产生的碱性的问题,从而给食品安全带来潜在的隐患。为了规抗革兰氏
8、阴性菌的多肽抗生素,对大肠杆菌、沙门氏范用药,本试验选择3个用药剂量(5mg/kg、菌等有很好的杀菌效果n]。在猪和鸡上的研究表10mg/kg和20mg/kg)配制溶液,并用生产中常用明,日粮中添加硫酸粘杆菌素能显著提高
此文档下载收益归作者所有