分子细胞遗传学常用实验技术.doc

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1、分子细胞遗传学常用实验技术GeneralLaboratoryGuidelinesA.Beforestarted:Beforeyouenterthelaboratory,makesureyouhavereadandfullyunderstandthelaboratorysafetymanual.Pleasereadthelaboratorymanualbelowandfollowtheguidelinesofeachexperiment.KeepinmindthatalltheguidelinesinthismanualareINGENERALbutnotforeverydetailsteps.

2、Makesureyouhavefullyunderstoodeachstepintheprotocolanditsreason.Pleaseaskyoursupervisorifyouhaveanythingunclear.B.Solutions-Ifyouuseasolutionandthevolumeislow(lessthan500ml),notifythepersonwhomakesit.Donotwaituntilthereisalmostnoneleft.Ifyouanticipateusingalotofaspecificsolution,youmaywanttomakesome

3、foryourself.Makesureallsolutionsarelabeledwithyourinitials,dateandthechemicalnameandconcentration.C.Pipettipsandmicrofugetubes-Afteryouuseaboxofpipettips,refillitandplaceitontheshelf.Whenseveralaccumulatetechnicianwillautoclavethemandreturntothedrawer.Keepyourplasticboxofmicrofugetubes.Youcanjustref

4、illyourboxasnecessary.Don’taccumulatemanypipetboxeswithoutrefillingthemorotherswon’thaveanytouse.D.Autoclave-Donotautoclavewasteitemswithsterileitems.E.Orders-Ifyouseethatsomethingisrunningloworifyouanticipateusingalotofanitem,notifytheresponsiblepersonandhewillplacetheorder.Donotwaituntiltheitemisg

5、oneoralmostgone-givetheorderinadvance.F.LaminarFlowHood-Makesureyousprayitdownwith70%ethanolbeforeandafteryouuseit.Ifthehazardouswasteisfilled-autoclaveit.Donotletitoverfill-thewasteshouldbedisposedofweekly.G.Dishes-Wehaveaverysmallsinksowashyourglasswareeveryday.Washwithsoapandwater.Rinsewithdistil

6、ledwater.Whenyouritemshavedried,putthemaway.Don’tletitemsaccumulatenearthesink.Thesamegoesforyourbench-cleanupaftereveryuse.H.Equipment-Ifyouhaveaproblemwithanyequipment,notifytheresponsiblepersonimmediately.Beforeusinganyequipment,askfordirectionsorreadthemanualfirst.A.Pipets-Donotsetpipetsoverthei

7、rspecifiedrange.Pipettingsolutionupanddownslowly.Takegreatcarewhenpipetschemicalslikechloroform.Refertothefactorywebsiteforinstructionsonhowtofixbrokenpipetsortheinstructionbooklet.P-20:2-20ulP-200:50