鞘内注射MrgC抗体对电针干预CFA大鼠慢性炎性痛及脊髓背角NR1表达的影响.pdf

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1、浙江中医药大学学撤2014年4月第38卷第4期鞘内注射MrgC抗体对电针干预CFA大鼠慢性炎性痛及脊髓背角NR1表达的影响张静若方芳方剑乔池细绿吴丽娜浙江中医药大学第三临床医学院杭州310053摘要：【目的】观察鞘内注射Mas—relatedGProtein-coupledC(MrgC)抗体对44(Electroacupuncture，EA)-'T--~J完全福氏佐剂(Complete?Freund"s?Ad—juvant，CFA)所致大鼠慢性炎性痛及脊髓背角(Spinaldorsalhorn，SDH)N一甲基一D一天门冬氨酸

2、受体亚基1(N—methyl—d—aspartatereceptors1。NRI)表达的影响。[方法1sI)雄性大鼠28只，脊髓鞘内插管成功后建立CFA模型，造模后随机分为生理盐水(Sahne)组、MrgC抗体(MrgCAb)组、MrgCAb+EA组和Saline+EA组，每组7只。两EA干预组从造模24h后在患侧“Y．_5-Y_”和“昆仑”穴开始电针刺激，参数为2／100Hz、30min，1次／d，连续7d，其余各组均做相应固定处理。造模后7d，各组大鼠鞘内注射相应试剂。在造模前和造模后24h、6d、7d检测大鼠患足热缩退潜

3、伏期(Them1alPawWithdrawalLatency，T—pwL)。免疫组织化学法检测各组大鼠患侧SDHNR1的表达。[结果1(1)各组大鼠在CFA造模前和造模后24h时，T—PWL均无明显差异。第6d时，与Saline组相比，MrgCAb+EA组和Saline+EA组大鼠患足T—PwL显著延长(P<0．O1)．MrgCAb组大鼠患足T—PWL无统计学差异。第7d时，与Saline组相比，MrgCAb组大鼠T—PwL明显缩短(尸<0．05)，而Saline+EA组显著延长(P<0．01)；与Saline+EA组比较，M

4、rgCAb+EA组大鼠T-PWL显著缩短(尸

5、005-5509(2014)04-0371-05IntrathecalInjectionofMrgCAntibodyInfluencestheEfectofElectroacupunctureonCFA-inducedChronicInflammatoryPainandNR1ExpressionofSpinalDorsalHorninRatsZhangJingruo。FangFang，FangJianqiao．etal7e7ClinicalMedicalCollegeofZhejiangChineseMedicalUniver

6、sity,Hangzhou(310053)Abstract：[Objective】Toobservewhethertheintrathecali~ecfionhasanimpactontheefectofelectroacupuncture(EA)interveningonchronicinflammatorypainofratsthatcausedbycompleteFreundsadjuvant(CFA1andontheexpressionofspinaldorsalhornN—methy1一d—aspartatesubu

7、nitl(NR1)l【Methods】SDrats28，eachfighthindfootofratswasmodeledwithCFAaftertheSuccessofthespinalcordsheathintubationAⅡratswererandomlydividedintofourgroups：saline，MrgCantibody，MrgCantibody+EAandsaline+EAEachgroupofseven．Detectthethermolpawwithdrawallatency(T—PWL)ofrat

8、s24hlater，chenstarttointerveneonthegroupsthatEAisincludedattwopointsofafectedside(”Zusauli”and”kunlun”)，2／100Hz，30min，onceadayandcontinuou