补阳还五汤对血管性痴呆大鼠海马CA1区LTP及ERK2与CaMKⅡβmRNA表达的影响.pdf

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1、中风与神经疾病杂志2013年1月第30卷第1期文章编号：1003—2754(2013)01-0011-04补阳还五汤对血管性痴呆大鼠海马CA。区LTP及ERK2与CaMKIIf3mRNA表达的影响唐敬龙，高维娟，钱涛，张泓波，李君摘要：目的观察补阳还五汤对血管性痴呆(VascularDementia，VD)大鼠海马CA。区突触传递LTP效应及对海马组织ERK2与CaMKII[3基因表达的影响。方法采用四血管阻断法建立大鼠VD动物模型。术后25d行水迷宫检测，30d采用在体大鼠海马CA侧枝一CA区长时程增强(LTP)记录方法记录海马CA区LTP。采用RT-PCR方法检测大鼠

2、海马CA．区脑组织ERK2与CaMKII[3mRNA表达。结果与模型组比较，补阳还五汤和尼莫地平组水迷宫实验平台象限游泳时间／游泳总时间、平台象限游泳距离／游泳总距离的比值显著增加，L腓’检测实验中兴奋性突触后电位(excitatorypostsynapticpotential，EPSP)斜率显著上升，大鼠海马ERK2与CaMKII[3mRNA表达显著增加。补阳还五汤与尼莫地平组相比，上述各指标均无显著差异。结论补阳还五汤可显著改善血管性痴呆大鼠学习记忆障碍，其作用可能与增强海马CA1区LTP，上调ERK2与CaMKII[3mRNA表达有关。关键词：补阳还五汤；血管性痴呆

3、；突触可塑性；长时程增强；ERK2andCaMKII[3；学习记忆中图分类号：R743．3文献标识码：AEffectofBYHWDonLTPandthemRNAexpressionofERK2andCaMKII[3inHippocampalCAlregionsofVDRatsTANGJing—long，GAOWei-juan，QIANTao，eta1．(HebeiChemicalandPharmaceuticalCollege，ShiJiazhuang050026，Chi—lza)Abstract：0bjectiveToinvestigatetheeffectsofBYH

4、WDontheIJTPofsynaptictransmissioninhippocampalCA．regionandthevariabilityofmRNAexpressionsofERK2andCaMKIII3inVDrats．ToexplorethemechanismofBYH—WDonimprovinglearningandmemory．MethodsRatswererandomlydividedinto4groups：Shamoperationgroup，VD+BYHWDgroup。VD+nimodipinegroupandVDgroup．TheratsofVD+

5、BYHWDgroup，VD+nimodipinegroupandVDgroupweremadebyfour-vesselocclusion．TheratsofVD+BYHWDgroupandVD+nimodipinegroupweredelivered-curedbyBYHWDandnimodipinebyintragastficadministrationafterawakeningfor30days．Morriswatermazetestswereusedtoobservethelearningandmemoryability．Thechangeofsynapticp

6、lasticityinhippocampusCA1regionofratsweredetected，HFSwasgivenbyCA3Schaffercollateral-commissuralpathway，andthenLTPofhippocampalCA1regionwasre—corded．RT．PCRwasusedtoobserveERK2andCaMKIII3mRNAexpressioninthebraintissue．ResultsWatermazede-tection：Thepercentofdistanceandtimebetweenthequadrant

7、previouslycontainingtheplatformwasshortenedsignificantly(P<0．05)．TheescapelatencyofratsofVD+BYHWDgroupandVD+nimodipinegroupweresignificantlyshorterascomparedwithModelgroup，andthepercentofdistanceandtimeinthequadrantpreviouslycontainingtheplatformwasin—creasedsignifi