补阳还五汤对血管性痴呆大鼠学习记忆和海马N-甲基-D-天冬氨酸受体表达的影响.pdf

《补阳还五汤对血管性痴呆大鼠学习记忆和海马N-甲基-D-天冬氨酸受体表达的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、·1316·中华老年心脑血管病杂志2012年12月第14卷第12期ChinJGeriatrHeartBrainVesselDis，Dec2012，Vol14，No．12基础研究．补阳还五汤对血管性痴呆大鼠学习记忆和海马N一甲基一D一天冬氨酸受体表达的影响李君，钱涛，高维娟，唐敬龙，魏涛，张青山，黄颖摘要：目的观察补阳还五汤对血管性痴呆(VaD)大鼠海马CA1区N一甲基I)_天冬氨酸受体表达及长时程增强(ITP)的影响及机制。方法选择SD大鼠144只，随机分为假手术组、VaD模型组(模型组)、尼莫地平组和补阳还五汤组，每组36只。采用四血管

2、阻断法制备VaD大鼠模型。Morris水迷宫实验评估大鼠学习记忆功能，大鼠海马cA3一cA1区记录海马cA1区LTP，免疫组织化学、Westernblot法和实时荧光定量PCR检测大鼠海马NR1、NR2A、NR2B蛋白和mRNA表达的变化。结果与假手术组比较，模型组大鼠逃避潜伏期延长和平均探索次数减少(P<0．05)，大鼠海马CA1区LTP明显降低(P<0．05)，CA1区NR1、NR2A、NR2B蛋白及mRNA表达水平明显降低(P<0．05)；与模型组比较，补阳还五汤组、尼莫地平组大鼠学习、记忆成绩明显提高(P<0．05)，海马cA1区

3、NR1、NR2A、NR2B蛋白及mRNA表达水平明显升高(P<0．05)。结论补阳还五汤可上调脑缺血再灌注海马CA1区脑组织NR1、NR2A、NR2B蛋白的表达，促进LTP，进而改善VaD大鼠学习记忆能力。关键词：补阳还五汤；痴呆，血管性；记忆障碍；海马；受体，N甲基一【)_天冬氨酸；聚合酶链反应EffectofBuyingHuanwuTangonlearning，memoryandexpressionofNMDAreceptorsinhippocampusofratswithvasculardementiaLIJun，QIANTao，G

4、AOWei—juan，etal(DepartmentofNeurology，XuzhouCentralHospital，Xuzhou221009，JiangsuProvince，China)Abstract：ObjectiveTostudytheeffectofBuyingHuanwuTang(DecoctionInvigoratingYangforRecuperation)onexpressionofNMDAreceptorsandLTPinhippocampalCA1areaofratswithvasculardementia(VaD

5、)anditsmechanism．MethodsOnehundredandforty—fourSDratswererandomlydividedintoshamoperationgroup，VaDmodelgroup，nimodipinegroup，andBuy—ingHuanwuTanggroup(36ineachgroup)．AratVaDmodelwasestablishedby4-vesselocclu—sion．LearningandmemoryoftheratswereassessedbyMorriswatermazetest

6、ingandrecordingtheLTPinhippocampalCA3一CA1area．ExpressionsofNR1，NR2AandNR2Bproteinsinhipp—ocampalneuronsweredetectedbyimmunohistochemistry，WesternblotandRT—PCR，respectively．ResultsTheescapinglatencytime(s)waslonger，theaveragenumberofcross—platformex—plorationwasless，theexp

7、ressionlevelsofNR1，NR2A，NR2BproteinsandmRNAandLTPinhippocampalCA1areaweresignificantlylowerinVaDmodelgroupthaninshamoperationgroup(P<0．05)．ThelearningandmemoryscoresweresignificantlyhigherinBuyingHuanwuTanggroupandnimodipinegroupthaninVaDmodelgroup(P<0．05)．Nosignificantdi

8、fferencewasfoundinlearningandmemoryscoresbetweenBuyingHuanwuTanggroupandnimodipinegroup．Conclusi