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时间:2020-05-24
《膨化人参对大鼠骨骼生长的影响及其机制初探.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、ChinJLabDiagn,Januai'y,2014,Vol18,No.1文章编号:1007—4287(2014)01—0014—04膨化人参对大鼠骨骼生长的影响及其机制初探卢焕俊,杨艳召,章小松。,许青松,刘丽萍,李范洙。,金元哲,李成福孙(1.延边大学长白山生物资源与功能分子教育部重点实验室(延边大学),延边大学医学院生理学与病理生理学教研部,吉林延吉133002;2.延边大学附属医院心血管外科,吉林延吉133000;3.延边大学农学院,吉林延吉133002)摘要:目的观察新型人参加工产品膨化人参对大鼠骨骼生长发育的影响,并初步探讨其可能的机制
2、。方法将32只SD大鼠,随机分为空白对照组和膨化人参低、中、高剂量组,每组8只。分别灌胃膨化人参悬浊液(0.75g/kg、1.5g/kg、3g/kg)和蒸馏水,连续灌胃3w。心脏采血后取股骨,测量股骨长度、直径、体积、股骨/体重比。利用三点弯曲生物力学实验,测量股骨最大弯曲应力、弯曲弹性模量、弯曲能量和弯曲韧性系数。检测血液中钙、磷和碱性磷酸酶。结果中剂量膨化人参组大鼠股骨/体重比、长度、直径、体积、血液中钙磷、碱性磷酸酶浓度以及生物力学性能指标均明显好于对照组(P3、性有关。’关键词:膨化人参;股骨;血钙;血磷;碱性磷酸酶;生物力学中图分类号:R336文献标识码:ATheeffectsofpuffginsengonthebonegrowthinratsanditsinitialmechanismLUHuan—jun,YANGYan—zhao,ZHANGXiao—song,eta1.(DepartmentofPhysiologyandPathophysiotogy,YanbianUniversityCollegeofMedicineandKeyLaboratoryofNaturalRexourcesofChangb4、aiMountain&FunctionalMolecules(YanbianUniversity)Min—istryofEducation,Yanji133002,China)Abstract:ObjectiveToobservetheeffectofanewginsengproducttwin—screwextrusionginseng(puffginseng)onthegrowthanddevelopmentofboneinratsanddiscussthepossibilitybiochemistrymechanism.Methods32SDr5、atsweredividedintocontrolgroup,puffginsenglow,middle,andhighdosegroups.8ratswereineachgroups.Theseratswerekeepingonintragastricadministrationofpuffginsengsuspension(O.75g/kg,1.5g/kg,3g/kg)anddistilledwa—terforthreeweeks.Aftercollectingthebloodofheart,choosethefemursandmeasureth6、elength,diameter,volumeofthefemursandtheboneweight/bodyweight.Thoughthe3-pointbendingtest,themaximumbendingstress,flexuralmodulus,bendingenergy,andflexuraltoughnesscoefficientofthefemursweremeasured.Theserumcalcium,phos—phorusandalkalinephosphataseweretested.ResultsThelength,di7、ameter,volumeofthefemurs,boneweight/bodyweight,serumcalcium,phosphorusandalkalinephosphataseandthefemursbiomechanicalcharacteristicsofratsinthemiddlepuffginsenggroupweresignificantlyhigherthanthecontrolgroup(P<0.05).ConclusionThepuffginsengcanpromotethegrowthanddevelopmentofbon8、einrats,whichmayberelevanttOtheincreaseofserumcalcium,
3、性有关。’关键词:膨化人参;股骨;血钙;血磷;碱性磷酸酶;生物力学中图分类号:R336文献标识码:ATheeffectsofpuffginsengonthebonegrowthinratsanditsinitialmechanismLUHuan—jun,YANGYan—zhao,ZHANGXiao—song,eta1.(DepartmentofPhysiologyandPathophysiotogy,YanbianUniversityCollegeofMedicineandKeyLaboratoryofNaturalRexourcesofChangb
4、aiMountain&FunctionalMolecules(YanbianUniversity)Min—istryofEducation,Yanji133002,China)Abstract:ObjectiveToobservetheeffectofanewginsengproducttwin—screwextrusionginseng(puffginseng)onthegrowthanddevelopmentofboneinratsanddiscussthepossibilitybiochemistrymechanism.Methods32SDr
5、atsweredividedintocontrolgroup,puffginsenglow,middle,andhighdosegroups.8ratswereineachgroups.Theseratswerekeepingonintragastricadministrationofpuffginsengsuspension(O.75g/kg,1.5g/kg,3g/kg)anddistilledwa—terforthreeweeks.Aftercollectingthebloodofheart,choosethefemursandmeasureth
6、elength,diameter,volumeofthefemursandtheboneweight/bodyweight.Thoughthe3-pointbendingtest,themaximumbendingstress,flexuralmodulus,bendingenergy,andflexuraltoughnesscoefficientofthefemursweremeasured.Theserumcalcium,phos—phorusandalkalinephosphataseweretested.ResultsThelength,di
7、ameter,volumeofthefemurs,boneweight/bodyweight,serumcalcium,phosphorusandalkalinephosphataseandthefemursbiomechanicalcharacteristicsofratsinthemiddlepuffginsenggroupweresignificantlyhigherthanthecontrolgroup(P<0.05).ConclusionThepuffginsengcanpromotethegrowthanddevelopmentofbon
8、einrats,whichmayberelevanttOtheincreaseofserumcalcium,
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