胶质瘤细胞中异柠檬酸脱氢酶1基因突变对肿瘤细胞的生长抑制及作用机制.pdf

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1、中华实验外科杂志2014年9月第31卷第9期ChinJExpSurg，September2014，Vo1．31，No．9·1857··申经夕卜科·胶质瘤细胞中异柠檬酸脱氢酶1基因突变对肿瘤细胞的生长抑制及作用机制左浩施金龙施炜倪兰春陈建【摘要】目的观察胶质瘤细胞中异柠檬酸脱氢酶1(IDH1)基因突变对肿瘤细胞的生长抑制作用并探讨其机制。方法构建表达IDH1“突变体的慢病毒载体，转染U87胶质瘤细胞，通过免疫荧光染色观察细胞增殖，原位缺口末端标记法(TUNEL)法检测细胞凋亡，水溶性四唑盐(WST)

2、法绘制细胞生长曲线，比色法检测细胞内还原性烟酰胺腺嘌呤二核苷酸磷酸(NADPH)、还原性谷胱甘肽(GSH)含量，荧光探针检测细胞内活性氧(ROS)含量；构建裸鼠动物模型，绘制肿瘤生长体积曲线与重量柱形图。结果表达IDH1“突变体的胶质瘤细胞生长明显抑制，免疫荧光染色以及TUNEL法证实细胞增殖减少、凋亡增多；细胞内NADPH水平降低伴随GSH含量降低和ROS蓄积，HO(1mmol／L)可明显增加胶质瘤细胞的生长抑制作用，GSH(1mmol／L)则可减弱抑制作用。结论IDH1基因R132H突变可显著

3、抑制胶质瘤细胞生长，细胞内GSH含量降低和ROS蓄积可能是其重要机制。【关键词】胶质瘤；异柠檬酸脱氢酶1；活性氧；还原性谷胱甘肽Growthinhibitoryefectofisocitratedehydrogenase1genemutationsingliomacelllineandpossi-blemechanismZuoHao，SJinlong，Js耽i，NiLanchun，ChenJian．DepartmentofNeurosurger-Y，AfiliatedHospitalofNanto

4、ngUniversity，Nantong226001，ChinaCorrespondingauthor：ShiJinlong，Emai．shi@foxmail．corn【Abstract】ObjectiveToexplorethegrowthinfluenceofisocitratedehydrogenasel(IDH1)mu—tationsongliomacellsandthemechanism．MethodsThelentivirusvectorexpressingIDH1R132Hmu—ta

5、ntgene．andtransfectedintoU87cells．TheproliferationofU87cellswasmeasuredwithproliferatingcel1nuclearantigen(PCNA)immunofluorescentstaining．andTdT—mediateddUTPnickendlabeling(TUNEL)methodwasusedtoevaluateapoptosis．ThegrowthcurveofU87cellswasdrawnbyusing

6、WSTassaykit．Cellularnicotinamideadeninedinucleotidephosphate(NADPH)andglutathione(GSH)levels．andintracellularreactiveoxygenspecies(R0S)weremeasuredbyfluorescencemicroplate．Aanimalmod—elofnudemicewasestablished．andthetumorvolumecurvesandthebarchartofth

7、eweightweredrawn．ResultsThegrowthwassignificantlyinhibitedingliomacellsoverexpressingIDH1“．Moreover．inthegliomacellsoverexpressingIDH1“．proliferationwasreduced．andapoptosiswasincreased．ThesecellswerecharacterizedbydecreasedintraeellularNADPHlevelsacco

8、mpaniedbyGSHdepletionandROSgen—eration．Accordingly，H，O，(1mmo1／L)couldobviouslyincreasetheinhibitionofmutantIDH1gliomaceilsgrowth．neverthelessGSH(1mmol／L)hadtheoppositeresult．Conclusion0urstudyprovidesdi—rectevidencethatR132HmutationofIDH1profo