不同超声参数对肿瘤细胞生长的抑制作用-论文.pdf

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1、·1504·任淑婷，等不同超声参数对肿瘤细胞生长的抑制作用不同超声参数对肿瘤细胞生长的抑制作用任淑婷，廖怡然，齐超，康小宁，张卉，赵兴华，李红丽，孙彭菲，王冰，TheinhbiflingefectofdiferentultrasoundparametersontumorcellgrowthRenShuting，LiaoYiran，QiChao，KangXiaoning，ZhangHui，ZhaoXinghua，LiHongli。，SunPengfei，WangBing’DepartmentofP

2、athology，SchoolofMedicine，Xi'anJiaotongUniversity，ShaanxiXi"an710061，China；TherapeuticVaccinesEngineer-ingCenterofShaanxiProvince，ShaanxiXi'an710061，China；DepartmentofPharmacology，SchoolofMedicine，Xi"anJiaotongUniversi—ty，ShaanxiXi"an710061，China；Xi"

3、anNorthCentralHospital，ShaanxiXi"an710032，China；ElementaryDepartment2，ShaanxiEner-gYInstitute，ShaanxiXi"an710613，China；。DepartmentofPharmacy，SchoolofMedicine，Xi"anJiaotongUniversity，ShaanxiXi"an710061，China；FirstAssociatedHospital，SchoolofMedicine，Xi

4、"anJiaotongUniversity，ShaanxiXi"an710061，China；WorkerHospital，the205thResearchInstitute，ChinaOrdnanceIndustry，ShaanxiXi"an710065，China．【Abstract】0bjective：Toexploretheeffectofdifferentultrasoundparametersontumorcellgrowthandprovidetheexperimentaldata

5、forultrasoundcombinedwithmicrobubbles—loadeddrugintumortherapy．Methods：Basesonthreefactorsoftheultrasonicintensity(1．48W／cm，2．56W／cm，2．79W／cm)，dutycycle(1：5，1：1，5：1)andul—trasonicexposuretime(5mins，10mins，15mins)，ninegroupsweredesignedbyorthogonalmet

6、hodsintheSKOV3celllineandMCF一7celllinewithdifferentultrasonictreatmentaswel1asthecontrolgroup(withoutanytreatment)wasdesigned．TheirgrowthinhibitionratewasevaluatedbyMTTassayandthesethreeparameterswereinturnanalyzed．Results：Comparedwiththecontrolgroup

7、，thecellulargrowthinhibitionwerenotobservedintwocelllinesatthedutycyclesof1：1and1：5．However，thecellulargrowthinhibitionratesweremorethan90％(P<0．05)inthreegroupsofSKOV3celllineatthedutycycleof5：1whileitwas48．8％．55．4％and88．7％inthreegroupsofMCF一7celllin

8、erespectively(尸<0．05)．Factorialanalysisshowedthreeultrasonicparametershadinteractioneffectonthecellu—larviabilityinSKOV3celllineandMCF一7cellline，butthedutycycleoftheuhrasoundwasthemostimportantfac—tot．Conclusion：Theroleofdifierentultrasoundparameters