罗格列酮对去卵巢大鼠学习记忆及脑内TNF-α、IL-6表达的影响.pdf

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1、MedicalScienceJournalofCentralSouthChina，January2014，Vo1．42，No．1文章编号：2095—1116(2014)o1—0040—04·基础医学·罗格列酮对去卵巢大鼠学习记忆及脑内TNF—O／．、IL一6表达的影响郑志民。，周寿红，胡弼(1．永州职业技术学院药理学教研室，湖南永州425006；2．南华大学医学院生理学教研室)摘要：目的观察罗格列酮对去卵巢大鼠学习记忆功能及海马内肿瘤坏死因子一OL(TNF·0【)和白介素-6(IL．6)表达的影响。方法56

2、只SD雌性大鼠，随机分为4组：假手术对照组、去卵巢组、假手术+罗格列酮组、去卵巢+罗格列酮治疗组，第15周进行Morris水迷宫试验。第16周处死大鼠，ELISA法检测大鼠大脑海马区的TNF—d、IL-6的表达。结果与假手术组比较，去卵巢组大鼠学习记忆能力明显下降(P<0．01)，大脑海马区TNF．OL和IL-6的表达明显升高(均P<0．01)；与去卵巢组比较，罗格列酮治疗组大鼠学习记忆能力明显改善，大脑海马区TNF一0【和IL-6的表达明显降低(均P<0．01)。结论罗格列酮能改善去卵巢大鼠的学习记忆障碍

3、，其机制可能与其降低海马TNF一0【和IL一6的表达有关。关键词：罗格列酮；去卵巢；学习记忆；TNF一0【；IL一6中图分类号：R963文献标识码：AEfectsofRosiglitazoneontheLearningandMemoryandExpressionofTumorNecrosisFactor．andInterleukin-6intheHippocampusinOvariectomizedRatsZHENGZhimin，ZHOUShouhong，HUBi(DepartmentofPharmacol

4、ogy，VocationalInstituteofTechnologyofYongzhou，YongzhouHunan425006，China)Abstract：ObjectiveToinvestigatetheeffectofrosiglitazoneonthelearningandmemo~andexpressionoftumornecrosisfactor一0【andinterleukin-6inthecerebruminovariectomizedrats．MethodsFifty—sixmaleS

5、prague—Dawleyratswererandomlydividedinto4groups：theshamcontrolgroup(SC)，ovariectomizedgroup(OV)，shamcontrol+rosiglitazonegroup(SC+R)andovariectomized+rosiglitazonegroup，(OV+R)．TheMorriswatermazetestwasperformedatthefif-teenthweek．Theratsweresacrificedatthe

6、sixteenthweek．Theexpressionsoftumornecrosisfactor—o【(TNF一)andinterleu—kin一6(IL-6)inthehippocampusweremeasuredbyELISAassay．ResultsComp~edwiththeSCgroup，theabilityoflearningandmemo~weresignificantlydecreasedandtheexpressionsofTNF一0【andIL一6inthehippocampuswer

7、esignificant—lyincreasedintheOVgroup(allP<0．01)．ComparedtheOVgroup，theabilityofmemo~wassignificantlyincreasedandtheexpressionsofTNF-6andIL一6inthehippocampusweresignificantlydecreasedintheOV+Rgroup(allP<0．01)．ConclusionRosiglitazoneimprovestheimpairmentofle

8、arningandmemo~inovariectomizedrats，themechanismofwhichmaybeassociatedwiththedown—regulationofTNF一andIL-6inthehippocampusinducedbyrosiglitazone．Keywords：Rosiglitazone；Ovariectomy；Learningandmemo~；TNF—ft．；IL一6女