黄芩苷对肝癌细胞株SMMC-7721裸鼠移植瘤生长抑制作用及其机制.pdf

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1、256董明，等黄芩苷对肝癌细胞株SMMC一7721裸鼠移植瘤生长抑制作用及其机铜黄芩苷对肝癌细胞株SMMC一7721裸鼠移植瘤生长抑制作用及其机制董明，侯俊明，高美花，魏辉ExperimentalstudyontheefectofBaicalinonCyclinD1andCaspase一3activityofhumanhepatocellularcarcinomaSMMC一7721celinnudemiceDongMing，HouJunming，GaoMeihua~，WeiHui、AfiliatedHospimlofShaan

2、xiUniversity。fChineseMedicine，ShaanxiXⅫ1712000，ChinaAffiliatedHospitalofYanbianUniversity，问inYanbian133000，China【Abstract】Objective：TotesttheefectofBaicalinongrowthoftransplantationtmnorusingtheSMMC一7721ani—malmodelinnudemicebearinghmnanhepatocellularcarcinonlacelll

3、ineMethods：BALB／cnudemicewereinjee—tedsubcutaneouslywithhmnanhepatolnacellineSMMC一77215×10／mlforduplicatetmnormodel20transplan—tednudemicewererandomlydividedintoblankcontrolgroupatdbaicalingroup，10ratsineachgroupThecontrolgroupwasgivennorlnalsalinebyintraperitoneali

4、njectionof10nfl／kg，Baicalinintraperitonealinjectionof100mkg，every2dinterlnitentdosingweightafter8tilnes，puttmnorweig，cancerinhibitionratetmnorCyclinD1andtheex—pressionofCaspase一3levelswerel~leasHredbyilnlnunohistochelnistryResults：Baicalin100mkgofthedorsmnSMMC一7721s

5、olidtmnoroflivercancerinhibitionratewas308％．theindexofliverandkidney．betweenthetwogroupswassimilar(P>005)，thesuppressionofCyclinD1expressionandraisedintheexpressionofCaspase一3(P<005)Condusion：BaicalininhumanliverSMMC一7721ofthedorsmnsubcutaneoustransplantationtmnorgr

6、owthinhibition，itsefectsmaycutCyclinD1expressionthroughorincreasetheexpressionofCaspase一3occlusiontoin—hibitproliferationandpromotingthefunctionofapoptosisintheprotectionofLllcorgansfunction．baicalinhasnoobviousadvantageBaicalinmaybecomeoneoftheefectivetreatlnentfnp

7、rimaryhepatocelularcarcinonlafHCC)【Keywords】Baicalin；humanhepatolnacellineSMMC一7721；CyclinD1；Caspase一3ModernOncology2014，22(02)：0256—0258【摘要】目的：以裸鼠人肝癌细胞株SMMC一7721移植瘤动物模型为研究对象，观察黄芩苷对移植瘤生长能力的影响，初步探讨黄芩苷对CyclinD1和Caspase一3表达的影响。方法：BALB／c裸鼠右前腋部皮下接种人肝癌SMMC一7721细胞5×10个，建立人

8、肝癌实体瘤模型。将20只成瘤裸小鼠随机分为空白对照组与黄芩苷组，每组l0只。对照组腹腔注射生理盐水10ml／kg，黄芩苷组腹腔注射黄芩苷注射液lOOmkg，每2d给药1次，间断给药8次后称体重、处死，瘤块离体称重，计算抑瘤率；用免疫组织化学方法检测实验组和对照组移植瘤中Cyc