谷胱甘肽对顺铂致小鼠染色体畸变的保护作用-论文.pdf

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1、中国细胞生物学学报ChineseJournalofCellBiology2013．35(10)：1492—1497DOI：10．11844／cjcb．2013．10．0146谷胱甘肽对顺铂致小鼠染色体畸变的保护作用安郁宽徐铭徐艳岩(滨州医学院基础学院，烟台264003；滨州医学院实验动物中心，烟~264003；。滨州I医学院药学院，烟台264003)摘要为了研究谷胱甘L-~(GSH)对顺铂(cDDP)所致染色体畸变的影响，将昆明小鼠随机分为空白对照组、GSH组、CDDP组、GSH+CDDP组进行实验GSH组按照1200mg／(kg·d1连续3Z尾静脉注

2、射GSH，对照组注射等量生理盐水：CDDP组于第2Z一次注射顺铂20mgg。对小鼠尾静脉取血检测血常规，处死小鼠，取骨髓进行有核细胞计数、微核实验和染色体G显带，双盲阅片并进行统计学分析。结果表对照组细胞染色体数目与形态完好，微核出现的机率极低。GSH组与空白对照组相比无显著差异>0．05)。CDDP组、GSH+CDDP组出现了明显的骨髓抑制现象，染色体畸变率及微核率显著增高，与空白对照组比较均有显著性差异(尸<0．01)。GSH+CDDP组与CDDP组相比染色体畸变率明显下降<0．05)。表明谷胱甘肽对顺铂所致的染色体损伤具有一定的保护作用。关键词谷

3、胱甘肽：顺铂：小鼠；染色体畸变TheProtectiveEffectofGlutathioneonChromosomeAberrationofMiceInducedbyCisplatinAnYukuan，XuMing，XuYanyan。(CollegeofBasicSciences，BinzhouMedicalUniversity,Yantai264003，China；ExperimentalAnimalCenter,BinzhouMedicalUniversity,Yantai264003，China；CollegeofPharmacy,Binzho

4、uMedicalUniversity,Yantai264003，China)AbstractTostudytheefectofglutathione(GSH)onchromosomeaberrationinducedbycisplatin(CDDP)，Kunmingmiceintheexperimentwererandomlydividedintothecontrolgroup，GSHgroup，CDDPgroup，andgroupofGSH+CDDEThemiceofGSHGroupwereinjectedwithGlutathionebytailv

5、eininaccordancewith1200mg／(kg。d)inthreedays．Thecontrolgroupwereinjectedwiththesamevolumeofnormalsaline．TheCDDPgroupwereinjectedoncewithCisplatinof20mg&gonthe2“day．Thebloodsamplewasdrawnbythetailveinofmiceforroutinebloodtest．Killthemiceandgetthebonemarrowsfornucleatedcellcount，mi

6、cronucleustestandchromosomeGbanding．Readtheslideswithdouble-blindmethodandcarryoutthestatisticalanalysis．Theresultsshowedthat，forthecontrolgroup，thenumbersandmorphologyofthechromosomewereregular,andthemicronucleuswasseldomobserved．ThecomparisonbetweentheGSHgroupandthecontrolgrou

7、pshowednodiferencefP>0．05)．ForthemiceofCDDPgroupandGSH+CDDPgroup，thebonemarowdepressionwasdistinctly,andthechromosomeaberationratesandmicronucleusratesweresignificantlyincreased，whichweresignificantlydiferentfromthecontrolgroup(尸<0．01)．TheaberrationrateintheGSH+CDDPgroupwassigni

8、ficantlylowerthanthatintheCDDPgroupfP<0．05)．The