鼠神经生长因子对丁胺卡那霉素耳毒性的保护作用.pdf

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1、听力学及言语疾病杂志2014年第22卷第5期507鼠神经生长因子对丁胺卡那霉素耳毒性的保护作用蔡琴芳蒋立新【摘要】目的探讨鼠神经生长因子(nervegrowthfactor，NGF)对丁胺卡那霉素(amikacin，AK)致豚鼠听力损伤的保护作用。方法45只豚鼠随机分为对照组、中毒组、治疗组三组，每组15只。对照组不予药物处理；中毒组腹腔注射AK400mg·kg·d_。，连续1O天；治疗组同法注射AK的同时肌肉注射NGF1500AU·-1·d一，连续14天。各组于给药前1天、给药结束后第1O、2O天分别行ABR测试，最后一次测试结束后处死所有豚鼠并取出听泡，行基底膜硝酸银染色铺片，观察耳

2、蜗毛细胞形态结构的变化及毛细胞缺失率。结果给药前三组豚鼠ABR反应阈差异无统计学意义(P>0．05)，给药结束后第1O、2O天中毒组ABR阈值均高于治疗组及对照组(P

3、013网络出版时间：2014—5—2015：45网络出版地址：http：／／www．cnki．net／kcms／doi／10．3969j／．issn．1O"O67299．2014．04．0．html【中图分类号】R764．5【文献标识码】A【文章编号】1006—7299(2014)05—0507—03ProtectiveEffectofMouseNerveGrowthFactoronAmikacin。——InducedOtotoxicityCaiQinfang，JiangLixin(DepartmentofOtoIaryngologyHeadandNeckSurgery，TheFirst

4、AfiliatedHospitalofJinanUniversity，Guangzhou，510630，China)[Abstract]ObjectiveToinvestigatetheeffectsofmousenervegrowthfactoronamikacin(AK)一inducedototoxicityinguineapigs．Methods45guineapigswererandomlydividedintothreegroupswith15guineapigsineachgroup．Thecontrolgroupreceivednodrugtreatment，thepois

5、oninggroupreceivedanintraperit0nealinjectionofAK(400mg·kg一·d)for10days，andthetreatmentgroupreceivedanintraperit0nea1injectionofAK(400mg·kg一·d一)for10daysandanintramuscularinjectionofNGF(1500AU·kg一·d一)for14consecutivedays．Auditorybrainstemresponsesweretestedonthedaybeforeadministration，the10thdayan

6、d20thdayafteradministration．AlltheguineapigsweresacrificedwhenthelastABRtestwasfinishedonthe20thdaywhoseCortiorganswereobservedaswel1．ResultsTheABRthresholdsofeachgroupshowednosignificantdifferencebeforeadministration．TheABRthresholdofthetreatmentgroupwaslowerthanthatofthepoisoninggroup．Withlight

7、microscopy，thehaircellswithnorma1morphologywereobservedinthecontrolgroup．However。therewasanex—tensive1OSSofouterhaircellinthepoisoninggroup．Forthetreatmentgroup，themissrateofhaircellwaslowercomparedwiththepoisoninggrou