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时间:2020-05-19
《实时荧光定量聚合酶链反应技术对结核性脑膜炎的诊断价值.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国实验诊断学2O15年1月第19卷第1期一35一文章编号:1007—4287(2015)01—0035—04实时荧光定量聚合酶链反应技术对结核性脑膜炎的诊断价值杨柳,苏明权,马越云,辛毅娟,韩若冰,张荣,文静,郝晓柯(第四军医大学西京医院全军医学检验中心,陕西西安710032)摘要:目的探讨实时荧光定量聚合酶链反应技术(RT—PCR)对结核性脑膜炎的诊断价值。方法分别采用涂片法、RT—PCR法对结核性脑膜炎、疑似结核性脑膜炎以及非结核性脑膜炎患者的脑脊液标本进行检测,并对检测结果进行比较分析。结果涂片法共检出1O例阳性,其中结核性脑膜炎7例
2、(70.00),疑似结核性脑膜炎3例(30.00);RT-PCR法共检出46例阳性,其中结核性脑膜炎31例(67.39),疑似结核性脑膜炎15例(32.61)。对结核性脑膜炎患者检测,涂片法阳性率(12.28)低于RT—PCR法阳性率(54.39Vo),差异有统计学意义(P3、有统计学意义(P4、jingHospitalofTheFourthMilitaryMedi—calUniversity,Xian,Shanxi,710032)Abstract:ObjectiveToinvestigatethediagnosisvalueofreal—timefluorescentquantitativepolymerasechainreac—tion(TR—PCR)intuberculousmeningitis.MethodsThecerebrospinalfluidspecimensofpatientswithtuberculousmenin5、gitis,suspectedtuberculousmeningitisandnon—tuberculousmeningitisweredetectedbyusingsmearmethodandRT—PCRrespectively,andthetesting?resultswerecomparedandanalyzed.ResultsTherewere10casesofpositivebysmearmethod,including7casesoftuberculousmeningitis(70.00)and3casesofsuspectedt6、uberculousmeningitis(30.oo);while46casesofpositivebyTR-PCRmethod,including31casesoftuberculousmeningitis(67.39)and15casesofsuspectedtuberculousmeningitis(32.61).Indetectionofpatientswithtuberculousmeningitis,theposi—tiveratebysmearmethodwas12.28,whichwaslowerthan54.39byRT—P7、CRmethod,thedifferencewasstatisti—callysignificance(P8、ingtuberculousmeningitiswas12.28,andtherateofmisseddiagno—siswas87.72,whiletheRT-P
3、有统计学意义(P4、jingHospitalofTheFourthMilitaryMedi—calUniversity,Xian,Shanxi,710032)Abstract:ObjectiveToinvestigatethediagnosisvalueofreal—timefluorescentquantitativepolymerasechainreac—tion(TR—PCR)intuberculousmeningitis.MethodsThecerebrospinalfluidspecimensofpatientswithtuberculousmenin5、gitis,suspectedtuberculousmeningitisandnon—tuberculousmeningitisweredetectedbyusingsmearmethodandRT—PCRrespectively,andthetesting?resultswerecomparedandanalyzed.ResultsTherewere10casesofpositivebysmearmethod,including7casesoftuberculousmeningitis(70.00)and3casesofsuspectedt6、uberculousmeningitis(30.oo);while46casesofpositivebyTR-PCRmethod,including31casesoftuberculousmeningitis(67.39)and15casesofsuspectedtuberculousmeningitis(32.61).Indetectionofpatientswithtuberculousmeningitis,theposi—tiveratebysmearmethodwas12.28,whichwaslowerthan54.39byRT—P7、CRmethod,thedifferencewasstatisti—callysignificance(P8、ingtuberculousmeningitiswas12.28,andtherateofmisseddiagno—siswas87.72,whiletheRT-P
4、jingHospitalofTheFourthMilitaryMedi—calUniversity,Xian,Shanxi,710032)Abstract:ObjectiveToinvestigatethediagnosisvalueofreal—timefluorescentquantitativepolymerasechainreac—tion(TR—PCR)intuberculousmeningitis.MethodsThecerebrospinalfluidspecimensofpatientswithtuberculousmenin
5、gitis,suspectedtuberculousmeningitisandnon—tuberculousmeningitisweredetectedbyusingsmearmethodandRT—PCRrespectively,andthetesting?resultswerecomparedandanalyzed.ResultsTherewere10casesofpositivebysmearmethod,including7casesoftuberculousmeningitis(70.00)and3casesofsuspectedt
6、uberculousmeningitis(30.oo);while46casesofpositivebyTR-PCRmethod,including31casesoftuberculousmeningitis(67.39)and15casesofsuspectedtuberculousmeningitis(32.61).Indetectionofpatientswithtuberculousmeningitis,theposi—tiveratebysmearmethodwas12.28,whichwaslowerthan54.39byRT—P
7、CRmethod,thedifferencewasstatisti—callysignificance(P8、ingtuberculousmeningitiswas12.28,andtherateofmisseddiagno—siswas87.72,whiletheRT-P
8、ingtuberculousmeningitiswas12.28,andtherateofmisseddiagno—siswas87.72,whiletheRT-P
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