芍药苷抑制大鼠小脑浦肯野细胞对急性缺氧的功能反应.pdf

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1、中国病理生理杂志ChineseJournalofPathophysiology2015，31(4)：664-668[文章编号]1000—4718(2015)04—0664—05芍药苷抑制大鼠小脑浦肯野细胞对急性缺氧的功能反应术任颖鸽，谭晓丽，陈静，张静，杜永平，张月萍(第四军医大学西京医院全军中医内科中心，儿科脑发育研究室，陕西西安710032)[摘要]目的：研究芍药苷(paeoniflorin，Pae)对大鼠小脑浦肯野细胞(Purkinjecells，PCs)急性缺氧电生理反应的影响。方法：采用全细

2、胞膜片钳记录法，记录大鼠小脑PCs膜电位、兴奋性和平行纤维(parallelfibre，PF)一Pc兴奋性突触后电流(excitatorypostsynapticcurrents，EPSCs)，观察急性缺氧和芍药苷对上述电生理功能的影响。结果：缺氧后PCs·首先表现为短暂的超极化，继之以短暂的去极化和持续超极化，芍药苷完全阻断了PCs的缺氧性超极化，并使PCs缺氧性去极化的幅度减小，持续时间缩短；缺氧上调了PCs兴奋性，芍药苷对缺氧引起的PCs的高兴奋性无显著影响；急性缺氧引起了PF．PCEPSCs的

3、长时程抑制(1ong．termdepression，LTD)；芍药苷可部分逆转缺氧引起的PF．PCEPSCs衰减。结论：芍药苷显著减轻了大鼠小脑PCs的缺氧反应，可能增强了PCs对急性缺氧的耐受性。[关键词]芍药苷；缺氧；浦肯野细胞；膜电位；突触传递[中图分类号]R363；R338．8[文献标志码]Adoi：10．3969／j．issn．1000-4718．2015．04．016PaeoniflorininhibitsfunctionalresponsesofratcerebellarPurkinje

4、cellstoacutehypoxiainsultRENYing—ge一，TANXiao．1．i．，CHENJing，ZHANGJing，DUYong—ping，ZHANGYue—ping(DepartmentofTraditionalChineseMedicine，LaboratoryofBrainDevelopment，DepartmentofPediatrics，XijingHospi—tal，TheFourthMilitaryMedicalUniversity，Xi’an710032，Chin

5、a．E-mail：ypzhang@fmmu．edu．cn；ddyypp@fmmu．edu．cn)．[ABSTRACT]AIM：Toinvestigatetheeffectsofpaeoniflorin(Pae)onthefunctionalresponsesinducedbyacutehypoxicinsultintheratcerebellarPurkinjecells(PCs)．METHODS：Thewhole—cellpatchclampwasusedfortheintra—cellularre

6、cordingofPCsintheratcerebellarslicestoevaluatethechangesofmembranepotential，theexcitabilityofPCs，andtheparallelfibre(PF)-PCexcitatorypostsynapticcurrents(EPSCs)uponacutehypoxicinsuhaloneorwiththepre·senceofPae．RESULTS：PCsshowedaninitialhyperpolarization

7、followedbybriefdepolarizationandlonglastingpost—hy—poxiahyperpolarizationafterhypoxiaexposure．Paecompletelyblockedhypoxia-inducedhyperpolarizationanddecreasedtheamplitudeandthedurationofhypoxicdepolarization．Hypoxiaup—regulatedtheexcitabilityofratPCs．Pa

8、edidn’tshowanysignificanteffectonthehypoxia—inducedhyperexcitabilityinPCs．Acutehypoxiainducedlong—termdepression(LTD)inratcerebellarPF—PCEPSCs，andPaepartiallyreversedhypoxia·induceddepressioninPF—PCEPSCs．CONCLUSION：Paesignificant