甲泼尼龙对大鼠急性脊髓损伤后髓鞘碱性蛋白的作用研究.pdf

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1、虫坐!喳匡盘查(鱼王版)2Q1生且簋8鲞簋22期JClinicians(ElectronicEdition),November15．2014．Vo1．8．No．22·93·基础论著甲泼尼龙对大鼠急性脊髓损伤后髓鞘碱性蛋白的作用研究董乐乐于泽周成江耿虹连建强肖友强王刚【摘要】目的探讨大剂量甲泼尼龙(MP)对急性脊髓损伤(ASCI)后大鼠脊髓组织和血清中的髓鞘碱性蛋白(MBP)的影响。方法6O只雄性SD大鼠，随机分成A组(正常对照组)、B组(实验对照组)、C组(治疗组)三组，每组2O只。A组单纯全椎板切除，不损伤脊髓

2、；B、C组采用Allen’s法制作T10脊髓损伤模型，C组首剂量以30mkg甲泼尼龙尾静脉注射，余剂量以5．4mg·kg"l-h-，每4h静脉给药一次，24h给完，A、B组以生理盐水代替甲泼尼龙尾静脉注射，剂量同c组。术后24h、48h对A、B、c三组大鼠后肢运动功能行BBB评分；再在各时间点取受损节段脊髓行HE染色及免疫组化染色观察形态学变化和MBP在脊髓组织中的分布特点；同时应用RT-PCR方法测定各组相应时间点脊髓组织中MBPmRNA表达量；取血清应用ELISA方法检测各组中血清MBP含量。结果C组大鼠在损

3、伤后24h、48h后肢运动功能恢复程度显著高于B组，差异有显著性(P<0．01)。B、C组血清中的MBP蛋白浓度较A组均明显升高，而C组血清的MBP蛋白浓度显著低于B组，差异有显著性(P<0．01)；C组脊髓组织中MBP表达高于B组，而低于A组，差异有显著性(尸<0．01)；C组MBPmRNA表达显著高于B组(户

4、；甲泼尼龙；MBPTheresearchoftheefectofMPOntheMBPofratssuferedfromASCIDongZele,YuZe,ZhouCheng／mng,GengHong,LianJianqiang,XiaoYouqiang,WangGang．DepartmentofOrthopedics,theFirstAfiliatedHospitalofBaotouMedicalCollege,Baotou014010,ChinaCorrespondingauthor：DongLele,Emai

5、l：gekdll@163．com[Abstract]0bjectiveTostudytheefectofhigh-dosemethylprednisolone(MP)onthemyelinbasicprotein(MBP)inmyoloidtissueandserurflofratssuferedfromacutespinalcordinjury(ASCI)．MethodsSixtySDmaleratswererandomlydividedintothreediferentgroups，eachofwhichha

6、d20rats．TheyweregroupA(normalcontrolgroup)，groupB(controlgroup)，andgroupC(treatmentgroup)．Rats’spineingroupAwasmerelyrespectedbyt0tallaminectomywithoutanyhurtstoitsspinalcord．BothgroupBandCwereassignedtobeevaluatedbyusingASCImodel(TIO)madebyAllen’Smethod．Rats

7、ingroupCwereinjectedtheMPwhichamountedto30mga~gatthebeginning．However,theremainingwereinjectedevery4hoursattheamountof5．4mg：kgeachtime．ThesamegoestogroupAandB，apartfromthatMEwhichwasreplaeedbynormalsalineinstead．After24hoursand48hoursoftheoperation，theresearc

8、herevaluateditshindlimbmovementfunctionofeachgroupbyvirtueofBBB．AndthenanalyzedthepathologicalchangesanddistributionfeatureofMBPbyobservingHE-stainingandimmunohistochemicalstainingofthein