脊髓小胶质细胞 P2 X4受体在 rrTNF 诱导的病理性疼痛中的作用.pdf

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1、·834·生国痘理生理杂ChineseJournalofPathophysiology2015，31(5)：834838[文章编号]1000-4718(2015)05—0834．05脊髓小胶质细胞P2X4受体在rrTNF诱导的病理性疼痛中的作用米宫庆娟，汪红华，梁颖，卢振和，陈金生，黄乔东，越宇(广州医科大学附属第二医院疼痛科，广东广州510260)[摘要]目的：研究P2X受体在坐骨神经周围给予重组大鼠TNF．(recombinantratTNF—d，rrTNF)引起的机械痛敏中的作用。方法：采用雄性sD大鼠(180～200g)，Westernblot检测坐骨神经周围给予“TNF后3d、7d

2、和14d脊髓背角P2X受体的表达水平，免疫荧光双染观察脊髓背角P2x4受体的表达部位；在坐骨神经周围给予rrTNF的大鼠前鞘内注射TNP．ATP，行为学检测大鼠50％机械撤足阈值的变化，Westernblot检测脊髓背角TNF—ot的表达是否发生变化。结果：与对照组相比，坐骨神经周围给予nTNF(100ng／L)后3d、7d和14d同侧脊髓背角P2X受体的表达水平明显升高(P<0．01)；P2X受体只表达于小胶质细胞中，神经元和星形胶质细胞中没有表达。坐骨神经周围给予rrTNF的大鼠前鞘内注射TNP-ATP可防止rrTNF诱导的机械痛敏并抑制脊髓背角TNF—ot的上调。结论：小胶质细胞P2X

3、受体可能通过上调脊髓背角的TNF—ot介导rrTNF诱导的机械痛敏。[关键词]P2X受体；肿瘤坏死因子ot；病理性疼痛；脊髓背角；小胶质细胞[中图分类号]R338．2[文献标志码]Adoi：10．3969／j．issn．1000—4718．2015．05．012EfectsofP2X4receptorinspinalmicrogliaonrrTNF·inducedpathologi-calpainGONGQing-juan，WANGHong-hua，LIANGYing，LUZhen—he，CHENJin—sheng，HUANGQiao—dong．YUEYu(DepartmentofPainM

4、edwine，TheSecondAfiliatedHospital，GuangzhouMedicalUniversity，Guangzhou510260，China．E—mail：qingjuangong@163．eom)[ABSTRACT]AIM：ToinvestigatetheeffectsofP2X4receptoronperi—sciaticadministrationofrecombinantratTNF．(rrTNF)一inducedmechanicalallodynia．METHODS：MaleSprague—Dawleyrats(180—200g)wereusedinthee

5、xperiments．ThelevelsofP2X4receptoronday3．day7andday14afterperi-sciaticadministrationofrrTNFwereexalTl—inedbyWesternblot，andthelocationofP2X4receptorinthespinaldorsalhornwasobservedbydoubleimmunofluores—cencestaining．Thechangesof50％paw—withdrawalthresholdsoftheratweredetectedbybehavioraltest，andthel

6、evelofTNF．0【inthespinaldorsalhornwasalsoexaminedbyWesternblotwhenTNP．ATPwasintrathecallyinjectedbeforethead—ministrationofrrTNF．RESULTS：Comparedwithcontrolgroup，theexpressionofP2X4receptorinthespinaldorsalhornontheipsilateralsidesignificantlyincreasedonday3，day7andday14(P<0．01)afterrrTNF(100ng／L)ad

7、ministra—tion．P2X4receptorwasco—localizedonlywithmieroglia，butnotwithneuronsorastrocytes．IntrathecalinjectionofTNP—ATPbeforerrTNFadministrationpreventedmechanicalallodyniainducedbyrrTNFandinhibitedtheupregu