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ID:55342733
大小:3.31 MB
页数:18页
时间:2020-05-14
《Allergic Disease final 过敏性疾病与抗体.ppt》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、AntigenselectioninthelgEresponseandallergicdisease.YanWangSupervisor:AndrewCollinsCosupervisor:WilliamSewellImmunoglobulinStructureofimmunoglobulinIsotypesofimmunoglobulinsImmunoglobulinDiversityGenerecombinationHeavychainvariableregion(VH)SomatichypermutationMutationleadstoloss
2、ofspecificity.CelldiesProgenyOriginalSelectedcellMutationleadstoimprovedbinding.CelldividesOthercellsAntigenSelectionYYYYYYYYXYYYYYYYYYYYYXDivisionsSHMDivisionsSHM→YYYYFunctionalBcellPlasmacellMemoryBcellPre-matureBcellIgEResponsesParasitedefencesAllergicdiseaseHygieneHypothesis
3、ofAllergicDiseasesGeneticsusceptibilityThehygienehypothesisstatesthatalackofearlychildhoodexposuretoinfectiousagentsincreasessusceptibilitytoallergicdiseases.HypothesisofIgEGenerationIgEsequencesarenotsubjectedtotheantigenselectionprocessesofthenormalgerminalcenterreactionIGHVge
4、nepolymorphismsAim:tocarefullydefinetheImmunoglobulinheavychainvariablegene(IGHV)repertoireIGHVgenesarereportedtobehighlypolymorphicArethereanymistakesintherepertoire?Mostallelesreportedbefore1995LackofunderstandingoftheorganizationofimmunoglobulingenelocusThesequencingtechnolog
5、ywerenotmatureMethodsCompilationofDatabase226IGHVpreviouslyreportedalleles4718rearrangedVDJgenesequencesfromEMBLBioinformaticanalysisThepublicationsthatreportedeachgermlinegenewerereviewedMultiplealignmentsofIGHVgermlinegeneAlignedgermlinegenestorearrangedsequencesbyiHMMune-alig
6、nprogramApparentmutationnumbersandpatternswereanalysedResultsFiveLevelsystemsforclassificationoftheIGHVgermlineallelesLevelDefinition1n=44Thereportedsequenceisarealallele.2n=38Thereportedsequenceisarealallele.Similaritiesbetweenthisalleleandotherknownallelesmeanthatmanyalignment
7、stothisallelearelikelytobeinerror.3n=23Thereportedsequencemaybearealallele.4n=17Thereportedsequencemaybearealallele.Similaritiesbetweenthisalleleandotherknownallelesmeanthatmanyalignmentstothisallelearelikelytobeinerror.5n=104Thereportedsequenceisunlikelytobearealallele.MostLeve
8、l5allelesareneverseenintherearrangedgenedatabas
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