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时间:2020-05-09
《靶向抑制 miR -19a 提高人宫颈癌 HeLa 细胞对顺铂敏感性的机制研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、崔立春,等靶向抑制miR一19a提高人宫颈癌HeLa细胞对顺铂敏感性的机制研究2014,14:31.grand),2013,Suppl59:L1876一L1881.蒋成英,戴广海.RasRafMekElk信号传导通路在肝细胞癌[15]HsufTr,uYC,ChiangIT,eta1.Sorafenibincreasesefficacyof发生中的作用机制及在靶向治疗中的应用[J].中国肿瘤临vorinostatagainsthumanhepatocelluIarcarcinomathroughtra
2、ns.·床,2008,35(23):1377—1380.duetioninhibitionofvorinastat——inducedERK/NF——kappaBsig-LinZH,WangL,ZhangJB,eta1.MST4promoteshepatocellularhaling[J].IntJOncol,2014,45(1):177—188.carcinomaepithelial—mesenchymaltransitionandmetastasisvia[16]FanC,ZhengW,FuX,e
3、ta1.StrategytoenhancethetherapeuticactivationoftheP—ERKpathway[J].IntJOncol,2014,45(2):effectofdoxorubicininhumanhepatocellularcarcinomabysele.-629—640.nocystine,asynergisticagentthatregulatestheROS—mediatedZhaoLY,ZhangJ,GuoB,eta1.MECP2promotescellprol
4、iferationsignaling[J].Oncotarget,2014,5(9):2853—2863.byactivatingERK1/2andinhibitingp38activityinhumanhepa—(编校:徐萌)tocellularcarcinomaHEPG2cells『J].CellMolBid(Noisy—le一靶向抑制miR一19a提高人宫颈癌HeLa细胞对顺铂敏感性的机制研究崔立春,杜建飞,耿会生,韩良辅,刘金鹏TargetedsuppressionofmiR——19oenh
5、ancedchemosensiUvityofcervicalcancerHeLacellstocisplatinCuiLichun,DuJianfei,GengHuisheng,HanLiangfu,LiuJinpengCancerCenter,Chang'anHo~tal:,ShaanxiXi包n710018,China.【Abstract】0bjegtive:ToinvestigatetheeffectofmiR一19aantisenseoligonucleotide(miR一19a—ASO)o
6、ncis—platinchemosensitivityinhumanHeLacellsanditsunderlyingmechanism.Methods:miR一19a—ASOwastransfect--edtoHeLacellsusingLipofeetamine2000.Real—timePCRwasusedtoevaluatethemiR一19aexpression.Cellvia--bilitywasmeasuredbymethyhhiazolyltetrazolium(MTF)assay.
7、TheexpressionofPTEN,AKT,andP—AKT(Se1473)proteinwasdetectedbyWesternblot.Results:miR一19a—ASO]inhibitedtheexpressionofmiR一19aef-fectively.HeLacellstransfectedwithmiR一19a—ASOwassensitizedtocisplatin(P<0.05)byMTrassay.Targe—tingtomiR一19acouldup—regulatePTE
8、N,anddown—regulateP—AKT(Ser473)expression.Conclusion:SilenceofmiR一19aincreasecellsensitivitytocisplatinprobablybysuppressingAKTsignalingpathway,providinganewtar-getforcervicalcancertherapy.【Keywords]cervicalcancer;chemosensitivity;miR一1
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