双抗原夹心ELISA法检测HCV抗体在无偿献血中的应用探讨-论文.pdf

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1、·968·北京医学2013年第35卷第11期·临床输血专栏．·论著．双抗原夹心ELISA法检测HCV抗体在无偿献血中的应用探讨刘保林林海玉z黄国永陈李1张小妹厉琳【摘要】目的探讨双抗原夹心ELISA法(简称夹心法)检测丙型肝炎病毒抗体(抗一HCV)在无偿献血中的应用方法对42份以往一种或两种间接法抗一HCV反应性的献血者标本及2831份无偿献血者标本同时采用一种夹心法和两种间接法试剂进行抗一HCV检测．对任一试剂检测为反应性的标本通过重组免疫印迹法(RIBA)作进一步确证。3种试剂检测均为阴性或RIBA结果为阴性判为真阴性．计算并对比各方法的特异

2、性。结果3种试剂检测均为阴性的标本2816份，均为反应性的标本32份．双试剂反应性标本7份，单试剂反应性标本18份。采用RIBA试剂对57份反应性标本进行检测，确认结果阳性26份、不确定12份、阴性19份。19份RIBA确认阴性的标本中，双抗原夹心法、某国产间接法、某进口间接法分别有1例、8例、l5例假阳性结果，特异性分别为99．96％(2834／2835)、99．72％(2827／2835)和99．47％(2820／2835)。结论夹心法诊断试剂的特异性高于间接法诊断试剂，为降低生物学假阳性．建议采用夹心法试剂检测抗一HCV。【关键词】丙型肝炎

3、病毒无偿献血双抗原夹心法间接法Detectionofanti-HCVantibodyusingdoubleantigensandwichELISAinvoluntaryblooddonationUUBao-[in．LINHai-yu．HUANGGuo-yong,etal(WenzhouBloodCenter,Wenzhou325007)【Abstract】ObjectiveToinvestigatethedoubleantigensandwichELISA(referredtoassandwichELISA)forde—teetionofhepa

4、titisCvirusantibody(anti-HCV)involuntaryblooddonation．MethodsOnesandwichELISAandtwoin—directELISAkitswereusedtomeasureanti——HCVin2831voluntarybloodsamplesand42voluntarybloodsamplespre-·viouslydetectedbyoneortwokindsofindirectELISAkits．Anti—HCV—positivesamplesdetectedbyanyofth

5、ethreekitswerefurtherconfirmedbyrecombinantimmunoblotassay(RIBA)．SampleswithnegativeresultsbyallthreekitsorwithnegativeresultsbyRIBAwereregardedaslillenegativeforanti-HCV．Results2816sampleswereallnegative，32sam—pieswereallpositivebythreekits，7sampleswerepositivebytwokits，and1

6、8sampleswerepositivebyonekit．Among57anti-HCV—positivesamples，26sampleswereRIBApositive，19sampleswereRIBAnegative，and12sampleswereRI—BAuncertain．Inthe19RIBAnegativesamples，1，8and15werepositivebythesandwichkit，thedomesticindirectkit，andtheimportedindirectkit，respectively．Thus，t

7、hespecificitiesforthesandwichkit，thedomestickitandtheimportedindirectkitwere99．96％(2834／2835)，99．72％(2827／2835)and99．47％(2820／2835)．ConclusionThespecificityofthesandwichELISAkitishigherthantheothertwoindirectELISAkits．Inordertoreducethebiologicalfalsepositiverate，thesandwichm

8、ethodissuggestedtobeusedtodetectanti-HCV．【Keywords】HepatitisCviresVo