CD4^＋T细胞三磷酸腺苷值及T细胞亚群测定在肝移植术后免疫功能监测中的应用-论文.pdf

《CD4^＋T细胞三磷酸腺苷值及T细胞亚群测定在肝移植术后免疫功能监测中的应用-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、中华实验外科杂志2014年4月第31卷第4期ChinJExpSurR，April2014，Vo1．31，No．4·843··临床研究·CD4+T细胞三磷酸腺苷值及T细胞亚群测定在肝移植术后免疫功能监测中的应用欧阳杰郭志勇马毅胡安斌朱晓峰何晓顺【摘要】目的探讨CD4T细胞三磷酸腺苷(ATP)值及T淋巴细胞亚群测定在肝移植术后免疫功能监测中的应用价值。方法采集48例行肝移植术后患者和30例健康志愿者的外周血样本，采用Immuknow免疫细胞功能测定试剂盒，检测CD4T细胞ATP值；流式细胞仪检测外周血T淋巴细胞亚群CD3、CD4、CD8细胞

2、的比例。结果正常人、稳定组、感染组与急性排斥组ATP值分别为(331±103)、(364±125)、(182±104)和(479±116)g／L，急性排斥组明显高于感染组(P<0．01)；稳定组与感染组比较，感染组明显降低(P<0．01)；稳定组与急性排斥组比较差异无统计学意义(P>0．05)。T淋巴细胞亚群检测结果，稳定组CD4细胞百分比为(33．23±l1．34)％，感染组为(25．53±6．89)％，两组比较差异有统计学意义(P

3、能。【关键词】肝移植；CD4T细胞；淋巴细胞亚群；免疫Applicationofadenosine-triphosphatevalueofCD4TcellsandTcellsubsetsanalysisinmonito-ringimmunefunctioninpost·livertransplantati0nrecipientsOuyangJie，GuoZhiyong，MaYi，HuAnbin，ZhuXiaofeng，Xiaoshun．DepartmentofTransplantationCenter，theFirstAffiliate

4、dHospitalofSunYat—senUniversitY．Guangzhou510080．inaCorrespondingauthor：ZhuXiaofeng，Emaif：740803277@qq．con【Abstract】ObjectiveToinvestigatethesignificanceofadenosine—triphosphate(ATP)valueofCD4TcellsandTcellsubsetanalysisinmonitoringimmunefunctionafterlivertransplantation．

5、MethodsImmuknowimmunecellfunctionkitwasappliedtotesttheATPvalHeofCIMTcellsinperipheralbloodfrom48patientsundergoinglivertransplantationand30healthyvolunteers．Additionally，thefrequencyofCD3．CIMandCD8cellsubsetswasanalyzedbyflowcytometry．ResuitsForhealthyvolunteers．stabler

6、ecipients．infectiousrecipientsandrecipientswithacutereiection，themeanATPlevelswere(331±103)，(364±125)，(182±l04)and(479±116)L，respectively．TheATPlevelsinacuterejectionpatientswerehigherthanstableones(P<0．O1)andpatientswithinfections(P<0．01)．How—ever，therewasnostatisticall

7、ysignificantdifiereneebetweenstablerecipientsandrecipientswithacutere．iection(P>0．05)．TcellsubsetsanalysisshowedthattheCD4celIcountswere(25．53±6．89)％intheinfectiouspatientsand(33．23±11．34)％instablegroup．therewasasignificantlydifferentintwogroups(P<0．05)．ConclusionTheATPl

8、evelsinCD4TcellsandTcellsubsetsanalysiscanindicatetheimmunestatusafterlivertransplantationtoacertainext