慢乙肝患者外周血中Th17细胞趋化因子受体的表达水平-论文.pdf

《慢乙肝患者外周血中Th17细胞趋化因子受体的表达水平-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、中华实验和临床病毒学杂志2014年6月第28卷第3期ChineseJExpClinVirol，June2014，Vo1．28，No．3·l61．论著艮乙肝患者外周血中Thl7细胞趋化因子受体的表达水平刘艳琼，杨小安，邹勇，李莉，潘兴飞，李刚，徐启桓【摘要】目的检测慢性乙型病毒性肝炎(chronichepatitisB，CHB)患者外周血中Thl7细胞表面趋化因子受体CCR4、CCR6、CXCR3的表达，分析CCR4、CCR6、CXCR3与丙氨酸转氨酶(ALT)、总胆红素(TBil)及HBVDNA载量的相关性。方法流式细胞仪检测30例CHB患者(CHB组)及l5名健

2、康人(对照组)外周血中Thl7细胞CCR4、CCR6、CXCR3的表达，与ALT、TBil、HBVDNA载量进行相关性分析。结果CHB组CD4+Thl7细胞CCR4、CCR6、CXCR3的表达水平高于对照组，其差异有统计学意义(P<0．05)。CHB组CD8+Thl7细胞CCR4、CCR6的表达水平高于对照组，其差异有统计学意义(P<0．05)。CCR4、CCR6表达水平与ALT、HBVDNA载量呈正相关(P<0．05)，与TBil水平无相关性(P>0．05)。结论CHB患者外周血中Thl7细胞表面CCR4、CCR6表达增高，与炎症程度相关，可能涉及Thl7细胞引

3、起的肝损伤。【主题词】肝炎，乙型，慢性；炎症趋化因子类ExpressionofchemokinereceptorsonThl7cellinperipheralbloodofpatientswithchronichepatitisBLiuYanqiong‘，YangXiaoan，ZouYong，LiLi，PanXingfei，LiGang，XuQihuan．DepartmentofInfectionsDeseases，TheThirdAffilatedHospitalofSunYatSenUniversity，Guangzhou510630，ChinaCorresp

4、ondingauthor：XUQihuan．Emaif：xqh03030513@aliyun．tom【Abstract】0bjectiveToinvestigatetheexpressionofchemokinereceptorsCCR4，CCR6，CXCR3onThl7cell，andanalyzethecorrelationbetweenchemokinereceptorsandserumbiochemicalparametersofliverfunctiontestandhepatitisBvirus(HBV)DNAloadinperipheralblood

5、ofchronichepatitisB(CHB)patients．MethodsThirtypatientswithCHB(CHBgroup)and15healthypersons(controlgroup)wereenrolledinthestudy．CCR4，CCR6，CXCR3expressionlevelsonThl7cellwereassayedbyflowcytometry．Thecorrelationbetweenchemokinereceptorsandalanineaminotransferase(ALT)，totalbilirubin(TBil

6、)，andHBVDNAloadwereanalyzedusingPearson’scorrelationanalysis，respectively．ResultsCCR4，CCR6，CXCR3expressionlevelsonCD4+Thl7cellinCHBgroupwerehigherthanthatofcontrolgroup．Moreover，thedifferencebetweenthemwasstatisticallysignificant(P<0．05)．CCR4，CCR6expressionlevelsonCD8+Thl7cellinCHBgro

7、upwerehigherthanthatofcontrolgroup(P<0．05)．AlthoughCXCR3expressionlevelonCD8+Thl7cellinCHBgroupwashigherthanthatofcontrolgroup，therewasnosignificantdifferencebetweenthem(P>0．05)．CCR4，CCR6expressionlevelswerepositivelycorrelatedwithserumALT，HBVDNAload，respectively(P<0．05)，butwerenotcor

8、relat