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时间:2020-05-04
《广西肝癌高发区蛋白质芯片联合ELISA筛检肝癌血清差异蛋白病例对照研究-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中华肿瘤防治杂志2013年1O月第2O卷第2o期CHINJCANCERPREVTREAT,October2013,Vo1.20No.20《流行病学研究/论著l广西肝癌高发区蛋白质芯片联合ELISA筛检肝癌血清差异蛋白病例对照研究何国珍黄天壬邓伟余家华张春燕利基林广西医科大学附属肿瘤医院实验研究部,广西南宁530021【摘要】目的:检测广西肝癌高发区肝细胞癌患者中血清相关差异蛋白。方法:应用基于广西肝癌高发地区为基础的病例对照研究方法,采用蛋白质芯片技术对广西肝癌高发区ll例肝癌患者与11名正常健康人血清蛋白进行检测,应用ELISA法对筛检出来的蛋白质在44例肝癌血清和44名正
2、常健康人血清中进行验证。结果:蛋白质芯片结果提示,肝癌组血清IGFBP一2的浓度为(887.48±225.91)pg/mL,高于对照组血清IGFBP一2浓度为(558.17±125.97)Dg/mL,t一2.8469,P一0.0216;肝癌组血清p21的浓度为(1040.97±395.91)pg/mL,低于对照组(2756.64±1355.64)Dg/mL,t一2.5706,P一0.0139;ELISA结果显示,肝癌患者血清IGFBP2浓度为(2006.54±407.71)ng/L,高于对照组(1463.81±634.70)ng/L,t一4.7723,P<0.O01;肝癌患者
3、血清p21浓度为(2293.095±1378.03)ng/I,低于对照组(3244.076士745.07)ng/L,£一4.0267,P一0.0001。结论:应用蛋白质芯片技术检测到的2个血清差异蛋白,可能是肝癌患者血清特异性生物标志,与广西肝癌高发区肝癌发生发展有关。【关键词】肝细胞癌;蛋白质芯片;ELISA;肿瘤标志中华肿瘤防治杂志,2Ol3,20(20):l553—1556Matchingcase-controlstudyonselectionandidentificationofdifferentialproteinsinserumwithhepatocellular
4、carcinamaintheendemicareaofHCCinGuangxibyproteinchipsandELISAHEGuo—zhen,HUANGTian—ten,DENGWei,YUJia—hua,ZHANGChun—yan。LIJi—linDepartmentofExperimentalResearch,CancerHospital,GuangxiMedicalUniversity,Nanning530021,P.R.ChinafABSTRACTloBJECTIVE:Todetectthedifferencesofrelativeserumproteinwithh
5、epatocellularcarcinomaintheen—demicareaofHCCinGuangxi.METHODS:Toapplymatchingcase—controlstudybasedontheendemicareaofHCCinGuangxi,theserumdifferentiallyexpressedproteinsofthepatientswithhepatocellularcarcinomaandthenormalhealtypeopleintheendemicareaofHCCweredetectedbytheproteinchipandvalida
6、tedbyELISA.RESULTS:1)Proteinchips:IGFBP一2levelofHCCandlevelofnormalhealtypeoplewere(887.48±225.91)og/mLand(558.17±125.97)pg/mL,respectively.ThelevelsofIGFBP一2inHCCwashigherthanthoseinnormalhealtypeople(一2.8469,P一0.0216);p21levelofHCCandlevelofnormalhealtypeoplewere(1040.974-395.91)pg/mLand(
7、2756.64_4-1355.64)pg/mL,respectively.Thelevelsofp21inHCCwaslowerthanthoseinnormalhealtypeople(t=2.5706,P一0.0139).2)ELISA:SerumIGFBP一2levelofHCCwas(2006.54-4-407.71)ng/LandSerumIGFBP一2levelinmatchednormalgroupswas(1463.81±634.70)ng/L.Statisticalanalysissh
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