小米谷糠多肽对肝损伤及癌前病变的抑制作用-论文.pdf

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1、※营养卫生食品科学2014,VoZ35,No．05213小米谷糠多肽对肝损伤及癌前病变的抑制作用于书佳，王常青术，原敏，连伟帅，訾艳，陈晓萌，郝志萍(山西大学生命科学学院，山西太原030006)摘要：目的：观察小米谷糠多肽的抗肿瘤和抑制化学性肝损伤作用。方法：分别用菠萝蛋白酶、木瓜蛋白酶、537酸性蛋白酶水解小米谷糠，得到3种多肽；先用噻唑蓝法检测3种谷糠多肽的体外抗肿瘤作用，然后用抗肿瘤效果最好的多肽喂食经氨基比林．NaNO诱导的肝损伤小鼠，并作肝组织学观察。结果：当菠萝蛋白酶水解的谷糠多肽质量浓度为50

2、mg／mL时，S180和H22肿瘤细胞的抑制率可达到56．28％和53．73％，该多肽在10(kg·d)剂量可显著降低小鼠肝脏丙二醛含量和血清谷草转氨酶、谷丙转氨酶活性(P

3、desonLiverInjuryandPrecancerousLesionsYUShu-jia，WANGChang-qing，YUANMin，LIANWei-shuai，ZIYan，CHENXiao-meng，HAOZhi—ping(CollegeofLifeScience，ShanxiUniversity，Taiyuan030006，China)Abstract：Objective：Thisstudywasaimedtoinvestigatetheantitumoractivityofmilletbran

4、polypeptides(MBPEs)andtheirinhibitoryefectonchemicallyinducedliverinjury．Methods：Threekindsofpolypeptideswereobtainedbyenzymatichydrolysisofmilletbranwithbromelain，papainand537acidprotease，respectively．MTTassaywasappliedtodetecttheinvitroantitumorefectofMB

5、PEs．Themicewithhepaticinjurychemicallyinducedbyaminopyrineandsodiumnitritewerefedwiththepolypeptidewiththestrongestantitumorefect，andtheliverwashistologicallyobserved．Results：TheinhibitoryratesofMBPE(50mg／mL)preparedwithbromelainonS180andH22tumorcellswere5

6、6．28％and53．73％，respectively．Inthehigh-doseMBPEgroup(10g／(kg’d))，theserumlevelsofaspartate~lsfminase(AS1『)andalaninetransflmin~e(ALa3andthelivercontentofmalondialdehyde(MDA)weresignifieanaydecreased(P<0．01)．Theaaivityofliverghllal~oneperoxidase(GSH-Px)wassi

7、gnificantlyincreased(尸<0．01)．Thehistologicalexaminationofmeliverfissileshowedthatfivercellswereseverelyinjured．However,themorphologyofhcpatocy~inthehigh-dosegroupwasbasicallynorma1．Conclusion：Thepolypeptidesfrommilletbranhydrolyzedbybromelainhavesignifican

8、tlyinhibitoryefectsonfiverinjuryandprecancerouslesions．Keywords：milletbranpolypeptide；aminopyrine；antiannor；liverinjury；hepaticprecancerouslesions中图分类号：TS201．4文献标志码：A文章编号：1002—6630(2014)05—0213—05doi：10．7506／