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时间:2020-05-04
《五味子水提物对微波辐射引起大鼠肝细胞氧化应激损伤的预防作用-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、第39卷第6期吉林大学学报(医学版)Vo1.39NO.62013年11月JournalofJilinUniversity(MedicineEdition)NOV.20131173[文章编号]1671—587X(2013)06—1173—04五昧子水提物对微波辐射引起大鼠肝细胞氧化应激损伤的预防作用李妍,刘玉莲,纪朋艳,李庆华,赵东海。,张巍(1.吉林医药学院生物化学与分子生物学教研室,吉林吉林132013;2.吉林医药学院生物化学与分子生物学实验室,吉林吉林132013;3.吉林医药学院病理学教研室,吉林吉林1
2、32013)[摘要]目的:探讨五味子水提取物预防给药对微波辐射引起的大鼠肝细胞氧化应激损伤的影响,阐明五昧子水提取物对微波产生的肝损伤的预防和保护作用。方法:36只正常成年Wistar大鼠随机分为正常对照组、微波辐射组和五味子水提取物预防给药组,每组l2只。五味子水提取物预防给药组大鼠给予五味子水提取物灌胃,5d后与微波辐射组均进行微波辐射,并在微波辐射后0和16h分别检测各组大鼠肝组织丙二醛(MDA)浓度和SOD活性,HE染色观察各组大鼠肝组织和细胞形态学。结果:与正常对照组比较,辐射后0h微波辐射组大鼠肝组
3、织MDA浓度和s0D活性均无明显变化;而辐射后16h微波辐射组大鼠肝组织MDA明显增加,SOD活性明显降低(
4、P5、;微波辐射;肝脏;大鼠,Wistar[中图分类号]R963[文献标志码]APreventiveeffect0fschisandraewaterextractonoxidativestressinjuryoflivercellsofratsinducedbymicrowaveradiationLIYan,I.IUYu—lian,jiPeng—yan,IAQing—hua,ZHAODong-hai。,ZHANGWei(1.DepartmentofBiochemicalandMolecularBiology,Jili6、nMedicalCollege,Jilin132013,China;2.LaboratoryofBiochemicalandMolecularBiology,JilinMedicalCollege,Jilin132013,China;3.DepartmentofPathology,JilinMedicalCollege,Jilin132013,China)Abstract:ObjectiveTostudytheinfluenceofschisandraewaterextractintheoxidativestr7、essi~uryoflivercellsoftheratsinducedbymicrowaveradiation,andtOclarifythepreventiveeffectofschisandraewaterextractonhepaticinjuryinducedbymicrowaveradiationinrats.Methods36normaladultWistarratswererandomlydividedintonormalcontrolgroup(一12),microwaveradiationg8、roup(n12),andschisandraewaterextractpreventiongroup(一12).Theratsinschisandraewaterextractpreventiongroupwerepretreatedindividuallywithschisandraewaterextractfor5dbeforemicrowaveradiation,aftermicrowaveradiationandschisandraewaterextracttreatment,theconcentra9、tionofMDAandtheactivityofSODoftherats0and16hafterradationinvariousgroupsweredetected,andthemorphologicalchangesofthelivertissueandlivercellswereobservedbyHEstaining.ResultsTheconcentrationofMDAa
5、;微波辐射;肝脏;大鼠,Wistar[中图分类号]R963[文献标志码]APreventiveeffect0fschisandraewaterextractonoxidativestressinjuryoflivercellsofratsinducedbymicrowaveradiationLIYan,I.IUYu—lian,jiPeng—yan,IAQing—hua,ZHAODong-hai。,ZHANGWei(1.DepartmentofBiochemicalandMolecularBiology,Jili
6、nMedicalCollege,Jilin132013,China;2.LaboratoryofBiochemicalandMolecularBiology,JilinMedicalCollege,Jilin132013,China;3.DepartmentofPathology,JilinMedicalCollege,Jilin132013,China)Abstract:ObjectiveTostudytheinfluenceofschisandraewaterextractintheoxidativestr
7、essi~uryoflivercellsoftheratsinducedbymicrowaveradiation,andtOclarifythepreventiveeffectofschisandraewaterextractonhepaticinjuryinducedbymicrowaveradiationinrats.Methods36normaladultWistarratswererandomlydividedintonormalcontrolgroup(一12),microwaveradiationg
8、roup(n12),andschisandraewaterextractpreventiongroup(一12).Theratsinschisandraewaterextractpreventiongroupwerepretreatedindividuallywithschisandraewaterextractfor5dbeforemicrowaveradiation,aftermicrowaveradiationandschisandraewaterextracttreatment,theconcentra
9、tionofMDAandtheactivityofSODoftherats0and16hafterradationinvariousgroupsweredetected,andthemorphologicalchangesofthelivertissueandlivercellswereobservedbyHEstaining.ResultsTheconcentrationofMDAa
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