HPLC测定不同配比麻黄汤水煎液中的麻黄碱和伪麻黄碱.pdf

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1、喜20l4，29(4)：433~435HPLC测定不同配比麻黄汤水煎液中的麻黄碱和伪麻黄碱曾勇，李睿，曾岑，王平，孟宪丽，韦婷，邱一行(成都中医药大学药学院中药材标准化教育部重点实验室，四川成都611137)摘要：目的采用HPLC测定麻黄汤组方变化时对麻黄碱和伪麻黄碱煎出量的影响。方法制备麻黄汤，用均匀实验设计的16个不同配比麻黄汤水煎液，采用HPLC法测定麻黄碱和伪麻黄碱的含量，计算其与麻黄单独煎煮相比的煎出率；采用逐步回归法分析处方组成对麻黄碱和伪麻黄碱煎出量的影响。结果所用HPLC测试方法分离效果

2、好，灵敏度高、重复性好，不同配比麻黄汤水煎液中麻黄碱和伪麻黄碱的含量有明显差异。结论麻黄碱和伪麻黄碱煎出量具有极显著的正相关性；杏仁能极显著地增加麻黄碱、伪麻黄碱在水煎液中的煎出量。关键词：麻黄汤；配比；麻黄碱；伪麻黄碱；高效液相色谱法中图分类号：R917文献标志码：A文章编号：1006—0103(2014)04—0433—03DOI：10．13375／j．cnki．wcjps．2014．04．027Determinationofephedrineandpseudoephedrineintheaqueo

3、usextractsofMahuangdecoctionwithdifferentratiosbyHPLCZENGYong，LIRui，ZENGCen，WANGPing，MENGXian—li，WEITing，QIUYi—hang(TheMinistryofEducationKeyLaboratoryofStandardizationofChineseHerbalMedicine，ChengduUniversityofTCM，Chengdu，Sichuan，611137P．R．China)Abstrac

4、t：OBJECTIVEToestablishaquantitativemethodforephedrineandpseudoephedrineintheaqueousextractofMahuangdecoction(MHD)byHPLC，andtoevaluatetheinfluenceoftheaqueousextractfromMHDwithdifferentr~iosonthedecoctingcontentsofephedrineandpseudoephedrinebyHPLCmethod．M

5、ETHODSAqueousextractsofMHDwith16differentratioswaspreparedviauniformexperimentaldesign，andthenthecontentsofephedrineandpseudoephedrineWasdeterminedbyHPLCmethod，andtherebycalculatedthedecoctingratesofephedrineandpseudoephedrineoftheseabove16formulasanddec

6、octionfromEphedraeherbaalone．Subsequently，theinfluenceofdifferentcompositionsofprescriptionsWasanalyzedonthedecoctingcontentsofephedrineandpseudoephedrinebystepwiseregressionmethod．RESULTSI-IPLCmethodinthisstudywasgoodintheaspectofseparableeffect，sensiti

7、vityandreproducibility．AndthecontentsofephedrineandpseudoephedrineoftheaqueousextractfromMahuangdecoctionwithdifferentratiosweresignificantdifferent．CONCLUSIONThereisahighlysignificantpositivecorrelationbetweenthedecoctingcontentsofephedrineandpseudoephe

8、drine．AndArmeniacaeal'nzLFlzmsemenCanobviouslyincreasethedeeoctingcontentsofephedrineandpseudoephedrineofMHD．Keywords：Mahuangdecoctiaon；Ratio；Ephedrine；Pseud0ephedrine；HPLCCLCnumber：R917Documentcode：AArticle1D：1006—0103(20