二化性家蚕滞育发动期间滞育性卵和非滞育性卵的烟酰胺腺嘌呤二核苷酸变化.pdf

《二化性家蚕滞育发动期间滞育性卵和非滞育性卵的烟酰胺腺嘌呤二核苷酸变化.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、5A,tC●e仃ee2013，39(1)：0047—0051ISSN0257—4799：CN32—1115／SE—mail：CYKE@chin~ourna1．net．cn二化性家蚕滞育发动期间滞育性卵和非滞育性卵的烟酰胺腺嘌呤二核苷酸变化王启龙姚金美司马杨虎赵林J(苏州大学基础医学与生物科学学院，江苏苏州215123)摘要为了探究家蚕滞育发动期间呼吸耗氧量下降与烟酰胺腺嘌呤二核苷酸(NAD)含量变化的关系，用高效液相色谱法和分光光度法分别检测在25℃条件下产下后12～72h的二化性家蚕滞育性卵与非滞育性卵中的还原型NAD(NADH)含量、氧化型NAD(NAD)含量、乳酸脱氢酶(

2、LDH)活性和胞质苹果酸脱氢酶(cMDH)活性。滞育性卵中的NADH+NAD含量、NADH／NAD值及LDH活性分别在产下后18、36、24h达到峰值，而cMDH活性处于波动中。在产下后24—72h，非滞育性卵中的NADH／NAD值极显著低于滞育性卵，而LDH和cMDH活性极显著高于滞育性卵。据此可以推测，滞育发动期间家蚕滞育性卵的NAD降解增强和NAD再生下降共同导致呼吸耗氧量下降。关键词家蚕；滞育发动；呼吸耗氧量；烟酰胺腺嘌呤二核苷酸；乳酸脱氢酶；胞质苹果酸脱氢酶中图分类号$881．2文献标识码A文章编号0257—4799(2013)01—0047—05ChangeofNi

3、cotinamideAdenineDinucleotideinDiapauseandNon-diapauseEggsofBivoltineBombyxmoriDuringDiapauseIniti-ationWANGQi—LongYAOJin—MeiSIMAYang—HuZHAOLin—Chuan(SchoolofBiologyandBas『cMedicineSciences，SoochowUniversity，SuzhouJiangsu215123，China)AbstractInordertoinvestigatetherelationshipbetweenreductio

4、nOfrespiratoryoxygenconsumptionandchangeofnicotinamideadeninedinucleotide(NAD)contentduringdiapauseinitiationofthesilkworm(Bombyxmori)，reducedNAD(NADH)content，oxidizedNAD(NAD)content，lactatedehydrogenase(LDH)activityandcytosolicmalatede—hydrogenase(cMDH)activityindiapauseandnon—diapauseeggso

5、fabivoltinesilkwormvarietyfrom12hto72hafterovipositionat25ocweredeterminedbyusinghighperformanceliquidchromatographicandspectrOphOtOmetricmeth—ods．NADH+NADcontent．NADH／NADvalueandLDHactivityindiapauseeggsreachedthepeakvalueat18，36and24hafterovipositionrespectively，whilecMDHactivityremainedfl

6、uctuated．At24tO72haEeroviposition，NADH／NADvalueinnon—diapauseeggswasextremelysignificantlylowerthanthatindiapauseeggs，whilethecontentsofLDHandcMDHinnon—diapauseeggswasextremelysignificantlyhigherthanthatindiapauseeggs．ItisinferredthattheenhancementofNADdegradationandthedeclineofNADregenerati

7、onindiapauseeggsjointlyleadtothereductionofrespiratoryoxygenconsumptionduringdiapauseinitiation．KeywordsBombyxmori；Diapauseinitiation；Respirat—oryoxygenconsumption；Nicotinamideadeninedinucleo—收稿日期：2012—08—15接受日期：2012一o9—15tide；LactatedehydrOgenase；